Nickel in PDB 1fwi: Klebsiella Aerogenes Urease, H134A Variant

All present enzymatic activity of Klebsiella Aerogenes Urease, H134A Variant:
3.5.1.5;

The structure of Klebsiella Aerogenes Urease, H134A Variant, PDB code: 1fwi was solved by M.A.Pearson, P.A.Karplus, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 2.00
Space group	I 21 3
Cell size a, b, c (Å), α, β, γ (°)	170.800, 170.800, 170.800, 90.00, 90.00, 90.00
R / Rfree (%)	17 / n/a

The binding sites of Nickel atom in the Klebsiella Aerogenes Urease, H134A Variant (pdb code 1fwi). This binding sites where shown within 5.0 Angstroms radius around Nickel atom.
In total only one binding site of Nickel was determined in the Klebsiella Aerogenes Urease, H134A Variant, PDB code: 1fwi:

Nickel binding site 1 out of 1 in the Klebsiella Aerogenes Urease, H134A Variant


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 1 of Klebsiella Aerogenes Urease, H134A Variant within 5.0Å range: probe atom residue distance (Å) B Occ C:Ni574 b:24.9 occ:1.00 O C:HOH724 2.1 15.6 1.0 O C:HOH723 2.2 15.5 1.0 O C:HOH726 2.3 29.8 1.0 OQ1 C:KCX217 2.3 18.4 1.0 ND1 C:HIS246 2.4 17.7 1.0 NE2 C:HIS272 2.6 8.4 1.0 CX C:KCX217 3.1 20.0 1.0 CD2 C:HIS272 3.2 12.6 1.0 CE1 C:HIS246 3.3 17.6 1.0 OQ2 C:KCX217 3.3 15.2 1.0 CG C:HIS246 3.4 16.2 1.0 O C:HOH725 3.7 26.0 1.0 CE1 C:HIS272 3.7 12.6 1.0 O C:GLY277 3.8 18.3 1.0 CB C:HIS246 3.8 13.4 1.0 OD2 C:ASP360 4.0 14.0 1.0 NE2 C:HIS219 4.1 17.7 1.0 OD1 C:ASP360 4.2 13.8 1.0 CD2 C:HIS219 4.2 15.0 1.0 NE2 C:HIS246 4.4 19.6 1.0 CG C:HIS272 4.4 12.8 1.0 NZ C:KCX217 4.4 20.6 1.0 CD2 C:HIS246 4.5 17.1 1.0 CG C:ASP360 4.5 13.1 1.0 ND1 C:HIS272 4.6 14.8 1.0 CD2 C:PHE271 4.7 12.8 1.0 C C:GLY277 4.8 17.7 1.0 CB C:PHE271 4.8 11.3 1.0

I.S.Park, L.O.Michel, M.A.Pearson, E.Jabri, P.A.Karplus, S.Wang, J.Dong, R.A.Scott, B.P.Koehler, M.K.Johnson, R.P.Hausinger. Characterization of the Mononickel Metallocenter in H134A Mutant Urease. J.Biol.Chem. V. 271 18632 1996.
ISSN: ISSN 0021-9258
PubMed: 8702515
DOI: 10.1074/JBC.271.31.18632