Nickel in PDB 1q99: Crystal Structure of the Saccharomyces Cerevisiae Sr Protein Kinsae, SKY1P, Complexed with the Non-Hydrolyzable Atp Analogue, Amp-Pnp

The structure of Crystal Structure of the Saccharomyces Cerevisiae Sr Protein Kinsae, SKY1P, Complexed with the Non-Hydrolyzable Atp Analogue, Amp-Pnp, PDB code: 1q99 was solved by B.Nolen, J.Ngo, S.Chakrabarti, D.Vu, J.A.Adams, G.Ghosh, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.95 / 2.11
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	72.413, 88.947, 134.822, 90.00, 90.00, 90.00
R / Rfree (%)	22 / 26

The binding sites of Nickel atom in the Crystal Structure of the Saccharomyces Cerevisiae Sr Protein Kinsae, SKY1P, Complexed with the Non-Hydrolyzable Atp Analogue, Amp-Pnp (pdb code 1q99). This binding sites where shown within 5.0 Angstroms radius around Nickel atom.
In total 2 binding sites of Nickel where determined in the Crystal Structure of the Saccharomyces Cerevisiae Sr Protein Kinsae, SKY1P, Complexed with the Non-Hydrolyzable Atp Analogue, Amp-Pnp, PDB code: 1q99:
Jump to Nickel binding site number: 1; 2;

Nickel binding site 1 out of 2 in the Crystal Structure of the Saccharomyces Cerevisiae Sr Protein Kinsae, SKY1P, Complexed with the Non-Hydrolyzable Atp Analogue, Amp-Pnp


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 1 of Crystal Structure of the Saccharomyces Cerevisiae Sr Protein Kinsae, SKY1P, Complexed with the Non-Hydrolyzable Atp Analogue, Amp-Pnp within 5.0Å range: probe atom residue distance (Å) B Occ A:Ni362 b:39.8 occ:1.00 NE2 A:HIS145 1.9 40.8 1.0 OE2 A:GLU151 2.0 28.3 1.0 NE2 A:HIS234 2.1 23.1 1.0 CE1 A:HIS145 2.8 39.9 1.0 CD2 A:HIS234 2.9 21.7 1.0 CD A:GLU151 3.0 28.8 1.0 CD2 A:HIS145 3.0 40.7 1.0 CE1 A:HIS234 3.3 22.8 1.0 OE1 A:GLU151 3.3 32.3 1.0 OH A:TYR153 3.4 18.3 1.0 ND1 A:HIS145 4.0 42.3 1.0 CZ A:TYR153 4.0 17.2 1.0 CG A:HIS145 4.1 41.4 1.0 O A:HOH2071 4.2 33.9 1.0 CG A:HIS234 4.2 22.7 1.0 O A:LYS235 4.3 23.0 1.0 ND1 A:HIS234 4.3 21.7 1.0 CG A:GLU151 4.3 29.1 1.0 CE2 A:TYR153 4.5 17.1 1.0 CA A:GLY236 4.6 28.4 1.0 CE1 A:TYR153 4.8 18.9 1.0 CD A:PRO237 4.9 32.2 1.0 CA A:ALA147 4.9 31.2 1.0

Nickel binding site 2 out of 2 in the Crystal Structure of the Saccharomyces Cerevisiae Sr Protein Kinsae, SKY1P, Complexed with the Non-Hydrolyzable Atp Analogue, Amp-Pnp


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 2 of Crystal Structure of the Saccharomyces Cerevisiae Sr Protein Kinsae, SKY1P, Complexed with the Non-Hydrolyzable Atp Analogue, Amp-Pnp within 5.0Å range: probe atom residue distance (Å) B Occ B:Ni363 b:30.1 occ:1.00 OE2 B:GLU151 1.9 23.2 1.0 CE1 B:HIS145 2.0 36.6 1.0 NE2 B:HIS234 2.2 19.9 1.0 NE2 B:HIS145 2.8 36.5 1.0 CD B:GLU151 2.9 25.2 1.0 CD2 B:HIS234 3.0 17.8 1.0 ND1 B:HIS145 3.1 36.8 1.0 OE1 B:GLU151 3.2 28.6 1.0 CE1 B:HIS234 3.3 19.1 1.0 OH B:TYR153 3.4 20.1 1.0 CD2 B:HIS145 4.0 37.9 1.0 CZ B:TYR153 4.1 14.8 1.0 CG B:HIS145 4.2 37.7 1.0 CG B:HIS234 4.2 19.0 1.0 CG B:GLU151 4.3 24.1 1.0 ND1 B:HIS234 4.3 16.4 1.0 O B:LYS235 4.5 21.4 1.0 O B:PRO146 4.6 34.6 1.0 CE2 B:TYR153 4.7 16.3 1.0 CE1 B:TYR153 4.7 15.4 1.0 CA B:ALA147 4.9 31.4 1.0 CA B:GLY236 4.9 23.8 1.0

B.Nolen, J.Ngo, S.Chakrabarti, D.Vu, J.A.Adams, G.Ghosh. Nucleotide-Induced Conformational Changes in the Saccharomyces Cerevisiae Sr Protein Kinase, SKY1P, Revealed By X-Ray Crystallography Biochemistry V. 42 9575 2003.
ISSN: ISSN 0006-2960
PubMed: 12911299
DOI: 10.1021/BI0344331