Nickel in PDB 1wn7: Crystal Structure of Archaeal Family B Dna Polymerase Mutant

All present enzymatic activity of Crystal Structure of Archaeal Family B Dna Polymerase Mutant:
2.7.7.7;

The structure of Crystal Structure of Archaeal Family B Dna Polymerase Mutant, PDB code: 1wn7 was solved by T.Kuroita, H.Matsumura, N.Yokota, H.Hashimoto, T.Imanaka, T.Inoue, Y.Kai, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	33.26 / 2.75
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	74.344, 111.131, 111.559, 90.00, 90.00, 90.00
R / Rfree (%)	22.6 / 30.6

The binding sites of Nickel atom in the Crystal Structure of Archaeal Family B Dna Polymerase Mutant (pdb code 1wn7). This binding sites where shown within 5.0 Angstroms radius around Nickel atom.
In total only one binding site of Nickel was determined in the Crystal Structure of Archaeal Family B Dna Polymerase Mutant, PDB code: 1wn7:

Nickel binding site 1 out of 1 in the Crystal Structure of Archaeal Family B Dna Polymerase Mutant


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 1 of Crystal Structure of Archaeal Family B Dna Polymerase Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Ni1001 b:41.6 occ:1.00 OD1 A:ASP141 2.0 38.4 1.0 O A:HOH2013 2.3 50.0 1.0 CG A:ASP141 2.8 36.9 1.0 OD2 A:ASP141 2.9 36.4 1.0 OD1 A:ASP215 4.0 31.5 1.0 O A:ILE142 4.0 42.3 1.0 OD2 A:ASP215 4.1 33.5 1.0 OE1 A:GLU143 4.2 55.4 1.0 OD2 A:ASP315 4.2 48.5 1.0 CB A:ASP141 4.2 35.3 1.0 N A:ILE142 4.4 39.4 1.0 O A:TYR209 4.4 31.9 1.0 CG A:ASP215 4.4 33.5 1.0 CD2 A:PHE214 4.7 29.3 1.0 CA A:ASP141 4.8 35.7 1.0 CE2 A:PHE214 4.9 27.2 1.0 C A:ILE142 4.9 42.5 1.0

T.Kuroita, H.Matsumura, N.Yokota, M.Kitabayashi, H.Hashimoto, T.Inoue, T.Imanaka, Y.Kai. Structural Mechanism For Coordination of Proofreading and Polymerase Activities in Archaeal Dna Polymerases J.Mol.Biol. V. 351 291 2005.
ISSN: ISSN 0022-2836
PubMed: 16019029
DOI: 10.1016/J.JMB.2005.06.015