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Nickel in PDB 1wu6: Crystal Structure of Reducing-End-Xylose Releasing Exo-Oligoxylanase E70A Mutant Complexed with Xylobiose

Enzymatic activity of Crystal Structure of Reducing-End-Xylose Releasing Exo-Oligoxylanase E70A Mutant Complexed with Xylobiose

All present enzymatic activity of Crystal Structure of Reducing-End-Xylose Releasing Exo-Oligoxylanase E70A Mutant Complexed with Xylobiose:
3.2.1.156;

Protein crystallography data

The structure of Crystal Structure of Reducing-End-Xylose Releasing Exo-Oligoxylanase E70A Mutant Complexed with Xylobiose, PDB code: 1wu6 was solved by S.Fushinobu, M.Hidaka, Y.Honda, T.Wakagi, H.Shoun, M.Kitaoka, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 31.39 / 1.45
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 53.300, 86.607, 87.711, 90.00, 90.00, 90.00
R / Rfree (%) 17.1 / 18

Nickel Binding Sites:

The binding sites of Nickel atom in the Crystal Structure of Reducing-End-Xylose Releasing Exo-Oligoxylanase E70A Mutant Complexed with Xylobiose (pdb code 1wu6). This binding sites where shown within 5.0 Angstroms radius around Nickel atom.
In total only one binding site of Nickel was determined in the Crystal Structure of Reducing-End-Xylose Releasing Exo-Oligoxylanase E70A Mutant Complexed with Xylobiose, PDB code: 1wu6:

Nickel binding site 1 out of 1 in 1wu6

Go back to Nickel Binding Sites List in 1wu6
Nickel binding site 1 out of 1 in the Crystal Structure of Reducing-End-Xylose Releasing Exo-Oligoxylanase E70A Mutant Complexed with Xylobiose


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 1 of Crystal Structure of Reducing-End-Xylose Releasing Exo-Oligoxylanase E70A Mutant Complexed with Xylobiose within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ni1400

b:9.0
occ:1.00
 OE1 A:GLU30 1.9 10.7 1.0
OE2 A:GLU27 2.1 10.3 1.0
CD A:GLU27 2.7 11.4 1.0
OE1 A:GLU27 2.7 11.5 1.0
CD A:GLU30 2.9 11.3 1.0
OE2 A:GLU30 3.2 12.6 1.0
CG A:GLU27 4.2 8.7 1.0
CG A:GLU30 4.2 11.0 1.0
O A:HOH1550 4.4 25.3 1.0
O A:HOH1457 4.5 14.8 1.0
CB A:GLU30 4.6 10.3 1.0
O A:HOH1508 4.8 18.8 1.0
CA A:GLU27 4.9 9.3 1.0

Reference:

S.Fushinobu, M.Hidaka, Y.Honda, T.Wakagi, H.Shoun, M.Kitaoka. Structural Basis For the Specificity of the Reducing End Xylose-Releasing Exo-Oligoxylanase From Bacillus Halodurans C-125 J.Biol.Chem. V. 280 17180 2005.
ISSN: ISSN 0021-9258
PubMed: 15718242
DOI: 10.1074/JBC.M413693200
Page generated: Wed Dec 16 01:16:15 2020

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