Nickel in PDB 1ziu: Crystal Structure of Nickel-Bound Engineered Maltose Binding Protein

The structure of Crystal Structure of Nickel-Bound Engineered Maltose Binding Protein, PDB code: 1ziu was solved by P.G.Telmer, B.H.Shilton, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	38.08 / 2.00
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	37.400, 43.970, 57.880, 101.15, 107.72, 101.90
R / Rfree (%)	20.5 / 24.3

The binding sites of Nickel atom in the Crystal Structure of Nickel-Bound Engineered Maltose Binding Protein (pdb code 1ziu). This binding sites where shown within 5.0 Angstroms radius around Nickel atom.
In total 2 binding sites of Nickel where determined in the Crystal Structure of Nickel-Bound Engineered Maltose Binding Protein, PDB code: 1ziu:
Jump to Nickel binding site number: 1; 2;

Nickel binding site 1 out of 2 in the Crystal Structure of Nickel-Bound Engineered Maltose Binding Protein


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 1 of Crystal Structure of Nickel-Bound Engineered Maltose Binding Protein within 5.0Å range: probe atom residue distance (Å) B Occ A:Ni500 b:31.4 occ:1.00 O A:HOH736 2.1 15.4 1.0 NE2 A:HIS63 2.2 20.5 1.0 NE2 A:HIS66 2.3 24.5 1.0 O A:HOH737 2.4 29.0 1.0 CD2 A:HIS66 3.0 21.8 1.0 CD2 A:HIS63 3.1 21.1 1.0 CE1 A:HIS63 3.3 23.2 1.0 CE1 A:HIS66 3.5 24.7 1.0 OD2 A:ASP65 3.8 27.3 1.0 NE1 A:TRP62 4.2 13.5 1.0 CG A:HIS66 4.2 21.8 1.0 CG A:HIS63 4.3 19.5 1.0 ND1 A:HIS63 4.3 20.4 1.0 OD1 A:ASP65 4.3 30.4 1.0 ND1 A:HIS66 4.5 22.9 1.0 CG A:ASP65 4.5 25.9 1.0

Nickel binding site 2 out of 2 in the Crystal Structure of Nickel-Bound Engineered Maltose Binding Protein


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 2 of Crystal Structure of Nickel-Bound Engineered Maltose Binding Protein within 5.0Å range: probe atom residue distance (Å) B Occ A:Ni722 b:28.8 occ:1.00 O A:HIS39 2.1 17.1 1.0 ND1 A:HIS39 2.2 21.6 1.0 CE1 A:HIS39 3.1 21.8 1.0 CG A:HIS39 3.2 20.3 1.0 C A:HIS39 3.3 17.8 1.0 CB A:HIS39 3.6 18.6 1.0 CA A:HIS39 4.0 17.7 1.0 O A:HOH673 4.0 20.0 1.0 NE2 A:HIS39 4.3 21.1 1.0 CD2 A:HIS39 4.4 21.6 1.0 N A:PRO40 4.4 17.9 1.0 OD1 A:ASP41 4.4 32.6 1.0 O A:HOH707 4.5 29.9 1.0 N A:HIS39 4.5 15.6 1.0 CA A:PRO40 4.7 19.2 1.0 C A:PRO40 4.7 20.6 1.0 O A:PRO40 5.0 20.4 1.0

P.G.Telmer, B.H.Shilton. Structural Studies of An Engineered Zinc Biosensor Reveal An Unanticipated Mode of Zinc Binding. J.Mol.Biol. V. 354 829 2005.
ISSN: ISSN 0022-2836
PubMed: 16288781
DOI: 10.1016/J.JMB.2005.10.016