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Nickel in PDB 260l: An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme

Enzymatic activity of An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme

All present enzymatic activity of An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme:
3.2.1.17;

Protein crystallography data

The structure of An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme, PDB code: 260l was solved by J.W.Wray, W.A.Baase, G.J.Ostheimer, B.W.Matthews, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.00 / 1.80
Space group P 32 2 1
Cell size a, b, c (Å), α, β, γ (°) 62.170, 62.170, 96.780, 90.00, 90.00, 120.00
R / Rfree (%) 19 / n/a

Other elements in 260l:

The structure of An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme also contains other interesting chemical elements:

Chlorine (Cl) 2 atoms

Nickel Binding Sites:

The binding sites of Nickel atom in the An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme (pdb code 260l). This binding sites where shown within 5.0 Angstroms radius around Nickel atom.
In total 2 binding sites of Nickel where determined in the An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme, PDB code: 260l:
Jump to Nickel binding site number: 1; 2;

Nickel binding site 1 out of 2 in 260l

Go back to Nickel Binding Sites List in 260l
Nickel binding site 1 out of 2 in the An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 1 of An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ni500

b:26.2
occ:1.00
O A:HOH501 1.9 17.8 1.0
O A:HOH502 1.9 19.2 1.0
NE2 A:HIS21 2.1 25.9 1.0
O A:HOH503 2.1 18.1 1.0
O A:HOH504 2.1 37.4 1.0
NE2 A:HIS142 2.3 10.6 1.0
CD2 A:HIS142 3.0 11.1 1.0
CD2 A:HIS21 3.1 23.8 1.0
CE1 A:HIS21 3.1 25.4 1.0
CE1 A:HIS142 3.4 21.1 1.0
OE2 A:GLU22 3.7 51.0 1.0
NE2 A:GLN105 3.9 20.8 1.0
O A:HOH232 4.1 39.6 1.0
ND1 A:HIS21 4.2 22.7 1.0
CG A:HIS21 4.2 19.8 1.0
CG A:GLU22 4.2 43.8 1.0
CG A:HIS142 4.3 13.8 1.0
O A:HOH207 4.4 45.3 1.0
ND1 A:HIS142 4.4 25.3 1.0
O A:HOH214 4.5 61.3 1.0
CD A:GLU22 4.6 60.9 1.0
O A:HOH224 4.6 33.1 1.0
O A:HOH209 4.6 47.3 1.0
OD2 A:ASP20 4.7 60.9 1.0

Nickel binding site 2 out of 2 in 260l

Go back to Nickel Binding Sites List in 260l
Nickel binding site 2 out of 2 in the An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 2 of An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ni600

b:30.5
occ:0.50
O A:HOH603 1.8 31.4 0.5
O A:HOH601 2.1 10.2 0.5
N A:MET1 2.1 32.7 1.0
O A:HOH602 2.2 17.1 0.5
CA A:MET1 3.0 24.6 1.0
OE1 A:GLU5 3.5 45.8 1.0
O A:HOH187 3.8 59.6 1.0
CB A:MET1 3.8 19.9 1.0
C A:MET1 4.1 24.2 1.0
O A:HOH188 4.3 62.1 1.0
O A:HOH223 4.4 29.2 1.0
CD A:GLU5 4.5 25.9 1.0
O A:MET1 4.6 17.1 1.0
OE2 A:GLU5 4.6 61.0 1.0
CE A:MET1 4.7 35.8 1.0

Reference:

J.W.Wray, W.A.Baase, G.J.Ostheimer, X.J.Zhang, B.W.Matthews. Use of A Non-Rigid Region in T4 Lysozyme to Design An Adaptable Metal-Binding Site. Protein Eng. V. 13 313 2000.
ISSN: ISSN 0269-2139
PubMed: 10835104
DOI: 10.1093/PROTEIN/13.5.313
Page generated: Wed Dec 16 01:16:42 2020

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