Nickel in PDB 2c21: Specificity of the Trypanothione-Dependednt Leishmania Major Glyoxalase I: Structure and Biochemical Comparison with the Human Enzyme
Protein crystallography data
The structure of Specificity of the Trypanothione-Dependednt Leishmania Major Glyoxalase I: Structure and Biochemical Comparison with the Human Enzyme, PDB code: 2c21
was solved by
A.Ariza,
T.J.Vickers,
N.Greig,
K.A.Armour,
I.M.Eggleston,
A.H.Fairlamb,
C.S.Bond,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
129.10 /
2.0
|
Space group
|
P 21 21 2
|
Cell size a, b, c (Å), α, β, γ (°)
|
130.193,
148.957,
50.698,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
15.5 /
20.1
|
Other elements in 2c21:
The structure of Specificity of the Trypanothione-Dependednt Leishmania Major Glyoxalase I: Structure and Biochemical Comparison with the Human Enzyme also contains other interesting chemical elements:
Nickel Binding Sites:
The binding sites of Nickel atom in the Specificity of the Trypanothione-Dependednt Leishmania Major Glyoxalase I: Structure and Biochemical Comparison with the Human Enzyme
(pdb code 2c21). This binding sites where shown within
5.0 Angstroms radius around Nickel atom.
In total 6 binding sites of Nickel where determined in the
Specificity of the Trypanothione-Dependednt Leishmania Major Glyoxalase I: Structure and Biochemical Comparison with the Human Enzyme, PDB code: 2c21:
Jump to Nickel binding site number:
1;
2;
3;
4;
5;
6;
Nickel binding site 1 out
of 6 in 2c21
Go back to
Nickel Binding Sites List in 2c21
Nickel binding site 1 out
of 6 in the Specificity of the Trypanothione-Dependednt Leishmania Major Glyoxalase I: Structure and Biochemical Comparison with the Human Enzyme
Mono view
Stereo pair view
|
A full contact list of Nickel with other atoms in the Ni binding
site number 1 of Specificity of the Trypanothione-Dependednt Leishmania Major Glyoxalase I: Structure and Biochemical Comparison with the Human Enzyme within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ni1142
b:35.4
occ:0.50
|
O
|
A:HOH2139
|
1.8
|
38.8
|
1.0
|
NE2
|
A:HIS8
|
2.1
|
37.3
|
1.0
|
OE1
|
B:GLU120
|
2.3
|
41.6
|
1.0
|
NE2
|
B:HIS77
|
2.3
|
35.8
|
1.0
|
OE1
|
A:GLU59
|
2.4
|
39.1
|
1.0
|
CE1
|
A:HIS8
|
3.1
|
40.0
|
1.0
|
CD2
|
A:HIS8
|
3.1
|
39.8
|
1.0
|
CE1
|
B:HIS77
|
3.2
|
35.5
|
1.0
|
CD
|
B:GLU120
|
3.3
|
41.8
|
1.0
|
CD2
|
B:HIS77
|
3.4
|
35.7
|
1.0
|
CD
|
A:GLU59
|
3.4
|
41.4
|
1.0
|
O
|
A:HOH2080
|
3.4
|
44.7
|
1.0
|
OE2
|
B:GLU120
|
3.8
|
46.5
|
1.0
|
OE2
|
A:GLU59
|
3.8
|
43.5
|
1.0
|
CB
|
B:ALA79
|
4.2
|
35.8
|
1.0
|
ND1
|
A:HIS8
|
4.2
|
37.1
|
1.0
|
CG
|
A:HIS8
|
4.2
|
38.0
|
1.0
|
O4
|
A:MRD2144
|
4.3
|
29.2
|
0.5
|
ND1
|
B:HIS77
|
4.3
|
35.3
|
1.0
|
CG
|
B:HIS77
|
4.5
|
35.3
|
1.0
|
CB
|
A:MET10
|
4.5
|
36.9
|
1.0
|
CG
|
B:GLU120
|
4.6
|
39.8
|
1.0
|
O4
|
A:MPD1144
|
4.7
|
30.4
|
0.5
|
CG
|
A:GLU59
|
4.7
|
38.5
|
1.0
|
O
|
B:HOH2157
|
4.8
|
44.9
|
1.0
|
CB
|
B:GLU120
|
4.8
|
37.8
|
1.0
|
CG
|
A:MET10
|
4.9
|
37.8
|
1.0
|
CB
|
A:GLU59
|
4.9
|
38.2
|
1.0
|
C5
|
A:MRD2144
|
4.9
|
29.5
|
0.5
|
|
Nickel binding site 2 out
of 6 in 2c21
Go back to
Nickel Binding Sites List in 2c21
Nickel binding site 2 out
of 6 in the Specificity of the Trypanothione-Dependednt Leishmania Major Glyoxalase I: Structure and Biochemical Comparison with the Human Enzyme
Mono view
Stereo pair view
|
A full contact list of Nickel with other atoms in the Ni binding
site number 2 of Specificity of the Trypanothione-Dependednt Leishmania Major Glyoxalase I: Structure and Biochemical Comparison with the Human Enzyme within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ni1143
b:36.4
occ:0.50
|
O
|
A:HOH2130
|
1.9
|
40.8
|
1.0
|
NE2
|
A:HIS77
|
2.2
|
35.1
|
1.0
|
OE1
|
A:GLU120
|
2.2
|
42.4
|
1.0
|
NE2
|
B:HIS8
|
2.3
|
40.0
|
1.0
|
OE1
|
B:GLU59
|
2.3
|
47.3
|
1.0
|
CE1
|
A:HIS77
|
3.0
|
36.7
|
1.0
|
CE1
|
B:HIS8
|
3.2
|
39.0
|
1.0
|
CD
|
A:GLU120
|
3.2
|
40.8
|
1.0
|
CD2
|
B:HIS8
|
3.3
|
38.6
|
1.0
|
CD2
|
A:HIS77
|
3.4
|
35.8
|
1.0
|
CD
|
B:GLU59
|
3.4
|
44.5
|
1.0
|
OE2
|
A:GLU120
|
3.6
|
44.4
|
1.0
|
OE2
|
B:GLU59
|
3.8
|
50.6
|
1.0
|
ND1
|
A:HIS77
|
4.2
|
37.4
|
1.0
|
CB
|
A:ALA79
|
4.3
|
37.0
|
1.0
|
ND1
|
B:HIS8
|
4.3
|
39.5
|
1.0
|
CG
|
A:HIS77
|
4.4
|
36.3
|
1.0
|
CG
|
B:HIS8
|
4.4
|
38.4
|
1.0
|
CB
|
B:MET10
|
4.5
|
37.8
|
1.0
|
CG
|
A:GLU120
|
4.5
|
39.5
|
1.0
|
CB
|
A:GLU120
|
4.6
|
38.0
|
1.0
|
CG
|
B:GLU59
|
4.7
|
40.0
|
1.0
|
CG
|
B:MET10
|
4.8
|
36.4
|
1.0
|
CB
|
B:GLU59
|
4.9
|
37.9
|
1.0
|
|
Nickel binding site 3 out
of 6 in 2c21
Go back to
Nickel Binding Sites List in 2c21
Nickel binding site 3 out
of 6 in the Specificity of the Trypanothione-Dependednt Leishmania Major Glyoxalase I: Structure and Biochemical Comparison with the Human Enzyme
Mono view
Stereo pair view
|
A full contact list of Nickel with other atoms in the Ni binding
site number 3 of Specificity of the Trypanothione-Dependednt Leishmania Major Glyoxalase I: Structure and Biochemical Comparison with the Human Enzyme within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Ni1142
b:32.5
occ:0.50
|
O
|
D:HOH2145
|
2.1
|
42.4
|
1.0
|
NE2
|
D:HIS77
|
2.3
|
36.0
|
1.0
|
OE1
|
D:GLU120
|
2.3
|
41.0
|
1.0
|
NE2
|
C:HIS8
|
2.3
|
34.3
|
1.0
|
OE1
|
C:GLU59
|
2.3
|
45.0
|
1.0
|
O
|
C:HOH2116
|
3.0
|
54.3
|
1.0
|
CE1
|
D:HIS77
|
3.1
|
34.8
|
1.0
|
CE1
|
C:HIS8
|
3.1
|
35.2
|
1.0
|
CD
|
D:GLU120
|
3.2
|
41.7
|
1.0
|
CD
|
C:GLU59
|
3.3
|
44.0
|
1.0
|
CD2
|
D:HIS77
|
3.4
|
36.1
|
1.0
|
CD2
|
C:HIS8
|
3.4
|
35.8
|
1.0
|
OE2
|
D:GLU120
|
3.6
|
42.6
|
1.0
|
OE2
|
C:GLU59
|
3.7
|
49.4
|
1.0
|
ND1
|
D:HIS77
|
4.3
|
35.5
|
1.0
|
ND1
|
C:HIS8
|
4.3
|
35.4
|
1.0
|
CB
|
D:ALA79
|
4.4
|
35.9
|
1.0
|
CG
|
D:HIS77
|
4.4
|
35.0
|
1.0
|
CG
|
C:HIS8
|
4.4
|
34.8
|
1.0
|
CB
|
C:MET10
|
4.5
|
37.0
|
1.0
|
CG
|
D:GLU120
|
4.5
|
39.4
|
1.0
|
CG
|
C:GLU59
|
4.7
|
40.2
|
1.0
|
CB
|
D:GLU120
|
4.7
|
37.9
|
1.0
|
CG
|
C:MET10
|
4.8
|
38.2
|
1.0
|
O
|
D:HOH2074
|
4.9
|
59.0
|
1.0
|
CB
|
C:GLU59
|
4.9
|
38.9
|
1.0
|
|
Nickel binding site 4 out
of 6 in 2c21
Go back to
Nickel Binding Sites List in 2c21
Nickel binding site 4 out
of 6 in the Specificity of the Trypanothione-Dependednt Leishmania Major Glyoxalase I: Structure and Biochemical Comparison with the Human Enzyme
Mono view
Stereo pair view
|
A full contact list of Nickel with other atoms in the Ni binding
site number 4 of Specificity of the Trypanothione-Dependednt Leishmania Major Glyoxalase I: Structure and Biochemical Comparison with the Human Enzyme within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Ni1143
b:29.8
occ:0.50
|
O
|
D:HOH2164
|
1.9
|
38.3
|
1.0
|
NE2
|
D:HIS8
|
2.2
|
37.5
|
1.0
|
NE2
|
C:HIS77
|
2.3
|
35.3
|
1.0
|
OE1
|
D:GLU59
|
2.4
|
40.6
|
1.0
|
OE1
|
C:GLU120
|
2.4
|
39.3
|
1.0
|
CD2
|
D:HIS8
|
3.1
|
38.9
|
1.0
|
CE1
|
C:HIS77
|
3.1
|
35.5
|
1.0
|
CE1
|
D:HIS8
|
3.2
|
38.4
|
1.0
|
CD2
|
C:HIS77
|
3.4
|
36.9
|
1.0
|
CD
|
D:GLU59
|
3.4
|
43.4
|
1.0
|
CD
|
C:GLU120
|
3.4
|
43.5
|
1.0
|
O
|
D:HOH2087
|
3.6
|
36.9
|
1.0
|
OE2
|
D:GLU59
|
3.8
|
43.2
|
1.0
|
OE2
|
C:GLU120
|
3.9
|
45.5
|
1.0
|
CB
|
C:ALA79
|
4.2
|
35.2
|
1.0
|
ND1
|
D:HIS8
|
4.3
|
37.7
|
1.0
|
CG
|
D:HIS8
|
4.3
|
37.7
|
1.0
|
ND1
|
C:HIS77
|
4.3
|
35.4
|
1.0
|
CB
|
D:MET10
|
4.4
|
36.7
|
1.0
|
O4
|
D:MRD1142
|
4.4
|
27.4
|
0.5
|
CG
|
C:HIS77
|
4.5
|
35.1
|
1.0
|
CG
|
C:GLU120
|
4.7
|
38.9
|
1.0
|
CG
|
D:GLU59
|
4.7
|
37.3
|
1.0
|
CB
|
C:GLU120
|
4.7
|
37.2
|
1.0
|
O4
|
D:MPD1141
|
4.8
|
30.7
|
0.5
|
O
|
C:HOH2190
|
4.8
|
47.5
|
1.0
|
CG
|
D:MET10
|
4.9
|
36.3
|
1.0
|
CB
|
D:GLU59
|
4.9
|
37.6
|
1.0
|
|
Nickel binding site 5 out
of 6 in 2c21
Go back to
Nickel Binding Sites List in 2c21
Nickel binding site 5 out
of 6 in the Specificity of the Trypanothione-Dependednt Leishmania Major Glyoxalase I: Structure and Biochemical Comparison with the Human Enzyme
Mono view
Stereo pair view
|
A full contact list of Nickel with other atoms in the Ni binding
site number 5 of Specificity of the Trypanothione-Dependednt Leishmania Major Glyoxalase I: Structure and Biochemical Comparison with the Human Enzyme within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
E:Ni1142
b:42.7
occ:0.50
|
O
|
E:HOH2061
|
2.0
|
60.6
|
1.0
|
OE1
|
F:GLU120
|
2.2
|
40.0
|
1.0
|
NE2
|
E:HIS8
|
2.3
|
34.1
|
1.0
|
NE2
|
F:HIS77
|
2.3
|
34.5
|
1.0
|
OE1
|
E:GLU59
|
2.4
|
44.4
|
1.0
|
CE1
|
F:HIS77
|
3.1
|
35.4
|
1.0
|
CE1
|
E:HIS8
|
3.2
|
34.8
|
1.0
|
CD
|
F:GLU120
|
3.3
|
40.3
|
1.0
|
CD2
|
E:HIS8
|
3.3
|
35.1
|
1.0
|
CD
|
E:GLU59
|
3.3
|
42.8
|
1.0
|
CD2
|
F:HIS77
|
3.5
|
35.1
|
1.0
|
OE2
|
E:GLU59
|
3.7
|
46.7
|
1.0
|
OE2
|
F:GLU120
|
3.7
|
43.3
|
1.0
|
CB
|
F:ALA79
|
4.3
|
37.5
|
1.0
|
ND1
|
F:HIS77
|
4.3
|
35.0
|
1.0
|
ND1
|
E:HIS8
|
4.3
|
34.0
|
1.0
|
CG
|
E:HIS8
|
4.5
|
34.5
|
1.0
|
CG
|
F:GLU120
|
4.5
|
39.6
|
1.0
|
CB
|
E:MET10
|
4.5
|
36.9
|
1.0
|
CG
|
F:HIS77
|
4.5
|
34.6
|
1.0
|
CB
|
F:GLU120
|
4.6
|
38.9
|
1.0
|
CG
|
E:GLU59
|
4.7
|
39.9
|
1.0
|
CG
|
E:MET10
|
4.8
|
36.5
|
1.0
|
CB
|
E:GLU59
|
4.9
|
38.5
|
1.0
|
|
Nickel binding site 6 out
of 6 in 2c21
Go back to
Nickel Binding Sites List in 2c21
Nickel binding site 6 out
of 6 in the Specificity of the Trypanothione-Dependednt Leishmania Major Glyoxalase I: Structure and Biochemical Comparison with the Human Enzyme
Mono view
Stereo pair view
|
A full contact list of Nickel with other atoms in the Ni binding
site number 6 of Specificity of the Trypanothione-Dependednt Leishmania Major Glyoxalase I: Structure and Biochemical Comparison with the Human Enzyme within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
E:Ni1143
b:46.8
occ:0.50
|
OE1
|
E:GLU120
|
2.0
|
35.4
|
1.0
|
O
|
F:HOH2024
|
2.1
|
54.8
|
1.0
|
OE1
|
F:GLU59
|
2.2
|
45.4
|
1.0
|
NE2
|
E:HIS77
|
2.2
|
34.9
|
1.0
|
NE2
|
F:HIS8
|
2.4
|
33.3
|
1.0
|
CD
|
E:GLU120
|
3.1
|
38.0
|
1.0
|
CE1
|
E:HIS77
|
3.1
|
34.6
|
1.0
|
O
|
E:HOH2105
|
3.1
|
51.4
|
1.0
|
CD
|
F:GLU59
|
3.1
|
46.3
|
1.0
|
CE1
|
F:HIS8
|
3.3
|
34.7
|
1.0
|
CD2
|
E:HIS77
|
3.3
|
35.5
|
1.0
|
CD2
|
F:HIS8
|
3.4
|
35.2
|
1.0
|
OE2
|
F:GLU59
|
3.4
|
49.7
|
1.0
|
OE2
|
E:GLU120
|
3.5
|
38.8
|
1.0
|
CB
|
E:ALA79
|
4.1
|
36.9
|
1.0
|
ND1
|
E:HIS77
|
4.2
|
34.3
|
1.0
|
CG
|
E:GLU120
|
4.3
|
37.4
|
1.0
|
CG
|
E:HIS77
|
4.4
|
35.5
|
1.0
|
ND1
|
F:HIS8
|
4.4
|
34.2
|
1.0
|
CG
|
F:HIS8
|
4.5
|
35.6
|
1.0
|
CB
|
F:MET10
|
4.5
|
37.0
|
1.0
|
CG
|
F:GLU59
|
4.5
|
43.1
|
1.0
|
O
|
F:HOH2009
|
4.6
|
57.5
|
1.0
|
CB
|
E:GLU120
|
4.6
|
37.0
|
1.0
|
CG
|
F:MET10
|
4.8
|
37.1
|
1.0
|
CB
|
F:GLU59
|
5.0
|
41.1
|
1.0
|
CD1
|
E:LEU122
|
5.0
|
35.6
|
1.0
|
|
Reference:
A.Ariza,
T.J.Vickers,
N.Greig,
K.A.Armour,
M.J.Dixon,
I.M.Eggleston,
A.H.Fairlamb,
C.S.Bond.
Specificity of the Trypanothione-Dependent Leishmania Major Glyoxalase I: Structure and Biochemical Comparison with the Human Enzyme. Mol.Microbiol. V. 59 1239 2006.
ISSN: ISSN 0950-382X
PubMed: 16430697
DOI: 10.1111/J.1365-2958.2006.05022.X
Page generated: Wed Oct 9 16:40:41 2024
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