Nickel in PDB 2noo: Crystal Structure of Mutant Nika

All present enzymatic activity of Crystal Structure of Mutant Nika:
3.6.3.24;

The structure of Crystal Structure of Mutant Nika, PDB code: 2noo was solved by C.Addy, M.Ohara, F.Kawai, A.Kidera, M.Ikeguchi, S.Fuchigami, M.Osawa, I.Shimada, S.Y.Park, J.R.H.Tame, J.G.Heddle, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.82 / 1.65
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	43.470, 92.248, 116.383, 90.00, 90.00, 90.00
R / Rfree (%)	17.7 / 21

The structure of Crystal Structure of Mutant Nika also contains other interesting chemical elements:

Iodine

(I)

6 atoms

The binding sites of Nickel atom in the Crystal Structure of Mutant Nika (pdb code 2noo). This binding sites where shown within 5.0 Angstroms radius around Nickel atom.
In total only one binding site of Nickel was determined in the Crystal Structure of Mutant Nika, PDB code: 2noo:

Nickel binding site 1 out of 1 in the Crystal Structure of Mutant Nika


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 1 of Crystal Structure of Mutant Nika within 5.0Å range: probe atom residue distance (Å) B Occ A:Ni1001 b:22.9 occ:1.00 O A:HOH1629 1.8 20.8 1.0 NE2 A:HIS56 2.0 15.4 1.0 NE2 A:HIS442 2.0 23.5 1.0 O A:HOH1631 2.1 27.5 1.0 O A:HOH1632 2.2 31.8 1.0 O A:HOH1630 2.2 29.1 1.0 CD2 A:HIS442 3.0 23.9 1.0 CD2 A:HIS56 3.0 16.4 1.0 CE1 A:HIS56 3.1 16.8 1.0 CE1 A:HIS442 3.1 23.8 1.0 CG A:HIS442 4.1 22.7 1.0 CG A:HIS56 4.1 14.4 1.0 ND1 A:HIS56 4.1 14.6 1.0 ND1 A:HIS442 4.2 26.3 1.0 O A:HOH1508 4.4 26.6 1.0 O A:HOH1627 4.6 42.8 1.0

C.Addy, M.Ohara, F.Kawai, A.Kidera, M.Ikeguchi, S.Fuchigami, M.Osawa, I.Shimada, S.Y.Park, J.R.Tame, J.G.Heddle. Nickel Binding to Nika: An Additional Binding Site Reconciles Spectroscopy, Calorimetry and Crystallography. Acta Crystallogr.,Sect.D V. 63 221 2007.
ISSN: ISSN 0907-4449
PubMed: 17242515
DOI: 10.1107/S0907444906048712