Nickel in PDB 3h85: Molecular Basis For the Association of Pipki Gamma-P90 with the Clathrin Adaptor Ap-2

All present enzymatic activity of Molecular Basis For the Association of Pipki Gamma-P90 with the Clathrin Adaptor Ap-2:
2.7.1.68;

The structure of Molecular Basis For the Association of Pipki Gamma-P90 with the Clathrin Adaptor Ap-2, PDB code: 3h85 was solved by A.Vahedi-Faridi, N.Kahlfeldt, J.G.Schaefer, V.Haucke, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	41.01 / 2.60
Space group	P 64
Cell size a, b, c (Å), α, β, γ (°)	125.301, 125.301, 74.546, 90.00, 90.00, 120.00
R / Rfree (%)	23.1 / 26.3

The binding sites of Nickel atom in the Molecular Basis For the Association of Pipki Gamma-P90 with the Clathrin Adaptor Ap-2 (pdb code 3h85). This binding sites where shown within 5.0 Angstroms radius around Nickel atom.
In total only one binding site of Nickel was determined in the Molecular Basis For the Association of Pipki Gamma-P90 with the Clathrin Adaptor Ap-2, PDB code: 3h85:

Nickel binding site 1 out of 1 in the Molecular Basis For the Association of Pipki Gamma-P90 with the Clathrin Adaptor Ap-2


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 1 of Molecular Basis For the Association of Pipki Gamma-P90 with the Clathrin Adaptor Ap-2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ni1 b:98.3 occ:0.50 CD A:LYS203 3.4 56.5 1.0 OH A:TYR205 3.5 55.3 1.0 NZ A:LYS203 3.8 56.0 1.0 CB A:LYS203 4.2 55.2 1.0 CE A:LYS203 4.2 55.7 1.0 CD2 A:PHE174 4.3 46.0 1.0 CG A:LYS203 4.4 55.9 1.0 CZ A:TYR205 4.5 54.6 1.0 CE2 A:PHE174 4.6 44.9 1.0 CG A:PHE174 4.6 47.3 1.0 CE2 A:TYR205 4.6 54.0 1.0 CB A:PHE174 4.9 49.1 1.0 OH P:TYR4 5.0 52.4 1.0

N.Kahlfeldt, A.Vahedi-Faridi, S.J.Koo, J.G.Schafer, G.Krainer, S.Keller, W.Saenger, M.Krauss, V.Haucke. Molecular Basis For Association of Pipki Gamma-P90 with Clathrin Adaptor Ap-2. J.Biol.Chem. V. 285 2734 2010.
ISSN: ISSN 0021-9258
PubMed: 19903820
DOI: 10.1074/JBC.M109.074906