Nickel in PDB 3pht: Crystal Structure of H74A Mutant of Helicobacter Pylori Nikr

The structure of Crystal Structure of H74A Mutant of Helicobacter Pylori Nikr, PDB code: 3pht was solved by E.Pozharski, S.Evans, S.Michel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	61.65 / 2.04
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	73.174, 73.174, 114.441, 90.00, 90.00, 90.00
R / Rfree (%)	19.1 / 23

The binding sites of Nickel atom in the Crystal Structure of H74A Mutant of Helicobacter Pylori Nikr (pdb code 3pht). This binding sites where shown within 5.0 Angstroms radius around Nickel atom.
In total 2 binding sites of Nickel where determined in the Crystal Structure of H74A Mutant of Helicobacter Pylori Nikr, PDB code: 3pht:
Jump to Nickel binding site number: 1; 2;

Nickel binding site 1 out of 2 in the Crystal Structure of H74A Mutant of Helicobacter Pylori Nikr


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 1 of Crystal Structure of H74A Mutant of Helicobacter Pylori Nikr within 5.0Å range: probe atom residue distance (Å) B Occ A:Ni149 b:25.4 occ:1.00 NE2 A:HIS99 2.1 25.5 1.0 ND1 A:HIS101 2.1 24.7 1.0 SG A:CYS107 2.2 27.6 1.0 CE1 A:HIS101 3.0 22.9 1.0 CD2 A:HIS99 3.0 22.4 1.0 CE1 A:HIS99 3.1 22.9 1.0 CG A:HIS101 3.1 22.6 1.0 CB A:CYS107 3.3 25.3 1.0 CA A:HIS101 3.4 23.3 1.0 CB A:HIS101 3.5 22.8 1.0 N A:HIS101 4.0 23.3 1.0 CA A:CYS107 4.0 25.8 1.0 NE2 A:HIS101 4.1 25.1 1.0 O A:ILE100 4.1 25.8 1.0 ND1 A:HIS99 4.2 24.2 1.0 CG A:HIS99 4.2 24.5 1.0 CD2 A:HIS101 4.2 24.2 1.0 C A:ILE100 4.3 25.8 1.0 O A:ASN106 4.7 26.7 1.0 C A:HIS101 4.7 27.5 1.0 N A:CYS107 4.8 25.4 1.0 CB A:ALA74 5.0 39.3 1.0

Nickel binding site 2 out of 2 in the Crystal Structure of H74A Mutant of Helicobacter Pylori Nikr


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 2 of Crystal Structure of H74A Mutant of Helicobacter Pylori Nikr within 5.0Å range: probe atom residue distance (Å) B Occ B:Ni149 b:33.5 occ:1.00 ND1 B:HIS101 2.0 28.0 1.0 NE2 B:HIS99 2.1 30.6 1.0 SG B:CYS107 2.2 34.5 1.0 CE1 B:HIS101 3.0 28.0 1.0 CD2 B:HIS99 3.1 26.5 1.0 CG B:HIS101 3.1 32.8 1.0 CE1 B:HIS99 3.1 28.3 1.0 CB B:CYS107 3.4 31.0 1.0 CA B:HIS101 3.5 33.7 1.0 CB B:HIS101 3.5 34.2 1.0 CA B:CYS107 4.0 33.1 1.0 N B:HIS101 4.1 32.2 1.0 NE2 B:HIS101 4.1 28.1 1.0 O B:ILE100 4.1 30.5 1.0 CD2 B:HIS101 4.2 30.1 1.0 ND1 B:HIS99 4.2 28.2 1.0 CG B:HIS99 4.2 29.1 1.0 C B:ILE100 4.4 30.2 1.0 O B:ASN106 4.6 37.5 1.0 N B:CYS107 4.7 34.3 1.0 C B:HIS101 4.8 37.9 1.0 CB B:ALA74 4.9 44.5 0.5

A.L.West, S.E.Evans, J.M.Gonzalez, L.G.Carter, H.Tsuruta, E.Pozharski, S.L.Michel. Ni(II) Coordination to Mixed Sites Modulates Dna Binding of Hpnikr Via A Long-Range Effect. Proc.Natl.Acad.Sci.Usa V. 109 5633 2012.
ISSN: ISSN 0027-8424
PubMed: 22451934
DOI: 10.1073/PNAS.1120283109