Nickel in PDB 4a8h: Crystal Structure of Putrescine Transcarbamylase From Enterococcus Faecalis with N-(Phosphonoacetyl)-Putrescine

All present enzymatic activity of Crystal Structure of Putrescine Transcarbamylase From Enterococcus Faecalis with N-(Phosphonoacetyl)-Putrescine:
2.1.3.6;

The structure of Crystal Structure of Putrescine Transcarbamylase From Enterococcus Faecalis with N-(Phosphonoacetyl)-Putrescine, PDB code: 4a8h was solved by L.M.Polo, F.Gil-Ortiz, V.Rubio, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.00 / 2.50
Space group	P 63 2 2
Cell size a, b, c (Å), α, β, γ (°)	117.200, 117.200, 225.345, 90.00, 90.00, 120.00
R / Rfree (%)	18.225 / 21.651

The binding sites of Nickel atom in the Crystal Structure of Putrescine Transcarbamylase From Enterococcus Faecalis with N-(Phosphonoacetyl)-Putrescine (pdb code 4a8h). This binding sites where shown within 5.0 Angstroms radius around Nickel atom.
In total 2 binding sites of Nickel where determined in the Crystal Structure of Putrescine Transcarbamylase From Enterococcus Faecalis with N-(Phosphonoacetyl)-Putrescine, PDB code: 4a8h:
Jump to Nickel binding site number: 1; 2;

Nickel binding site 1 out of 2 in the Crystal Structure of Putrescine Transcarbamylase From Enterococcus Faecalis with N-(Phosphonoacetyl)-Putrescine


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 1 of Crystal Structure of Putrescine Transcarbamylase From Enterococcus Faecalis with N-(Phosphonoacetyl)-Putrescine within 5.0Å range: probe atom residue distance (Å) B Occ A:Ni1346 b:20.9 occ:0.33 NE2 A:HIS69 2.2 16.7 1.0 O A:HOH2050 2.2 3.2 1.0 CE1 A:HIS69 3.2 17.2 1.0 CD2 A:HIS69 3.3 15.7 1.0 OE2 A:GLU60 4.2 15.3 1.0 O A:GLY70 4.2 15.4 1.0 ND1 A:HIS69 4.3 16.4 1.0 CG A:HIS69 4.4 15.0 1.0 O A:HOH2048 4.6 2.3 1.0

Nickel binding site 2 out of 2 in the Crystal Structure of Putrescine Transcarbamylase From Enterococcus Faecalis with N-(Phosphonoacetyl)-Putrescine


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 2 of Crystal Structure of Putrescine Transcarbamylase From Enterococcus Faecalis with N-(Phosphonoacetyl)-Putrescine within 5.0Å range: probe atom residue distance (Å) B Occ B:Ni1345 b:18.9 occ:0.33 O B:HOH2046 2.1 2.7 1.0 NE2 B:HIS69 2.3 13.8 1.0 CE1 B:HIS69 3.2 14.6 1.0 CD2 B:HIS69 3.3 12.8 1.0 OE2 B:GLU60 4.2 12.8 1.0 O B:GLY70 4.3 10.5 1.0 ND1 B:HIS69 4.3 13.7 1.0 CG B:HIS69 4.4 12.0 1.0 O B:HOH2049 4.6 11.3 1.0

L.M.Polo, F.Gil-Ortiz, A.Cantin, V.Rubio. New Insight Into the Transcarbamylase Family: the Structure of Putrescine Transcarbamylase, A Key Catalyst For Fermentative Utilization of Agmatine Plos One V. 7 31528 2012.
ISSN: ISSN 1932-6203
PubMed: 22363663
DOI: 10.1371/JOURNAL.PONE.0031528