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Nickel in PDB 4a8t: Crystal Structure of Putrescine Transcarbamylase From Enterococcus Faecalis Lacking Its C-Terminal Helix, with Bound N5-(Phosphonoacetyl)-L-Ornithine

Enzymatic activity of Crystal Structure of Putrescine Transcarbamylase From Enterococcus Faecalis Lacking Its C-Terminal Helix, with Bound N5-(Phosphonoacetyl)-L-Ornithine

All present enzymatic activity of Crystal Structure of Putrescine Transcarbamylase From Enterococcus Faecalis Lacking Its C-Terminal Helix, with Bound N5-(Phosphonoacetyl)-L-Ornithine:
2.1.3.6;

Protein crystallography data

The structure of Crystal Structure of Putrescine Transcarbamylase From Enterococcus Faecalis Lacking Its C-Terminal Helix, with Bound N5-(Phosphonoacetyl)-L-Ornithine, PDB code: 4a8t was solved by L.M.Polo, F.Gil-Ortiz, V.Rubio, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 30 / 1.59
Space group P 63 2 2
Cell size a, b, c (Å), α, β, γ (°) 89.990, 89.990, 184.320, 90.00, 90.00, 120.00
R / Rfree (%) 16.218 / 18.015

Nickel Binding Sites:

The binding sites of Nickel atom in the Crystal Structure of Putrescine Transcarbamylase From Enterococcus Faecalis Lacking Its C-Terminal Helix, with Bound N5-(Phosphonoacetyl)-L-Ornithine (pdb code 4a8t). This binding sites where shown within 5.0 Angstroms radius around Nickel atom.
In total only one binding site of Nickel was determined in the Crystal Structure of Putrescine Transcarbamylase From Enterococcus Faecalis Lacking Its C-Terminal Helix, with Bound N5-(Phosphonoacetyl)-L-Ornithine, PDB code: 4a8t:

Nickel binding site 1 out of 1 in 4a8t

Go back to Nickel Binding Sites List in 4a8t
Nickel binding site 1 out of 1 in the Crystal Structure of Putrescine Transcarbamylase From Enterococcus Faecalis Lacking Its C-Terminal Helix, with Bound N5-(Phosphonoacetyl)-L-Ornithine


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 1 of Crystal Structure of Putrescine Transcarbamylase From Enterococcus Faecalis Lacking Its C-Terminal Helix, with Bound N5-(Phosphonoacetyl)-L-Ornithine within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ni1319

b:9.0
occ:0.30
O A:HOH2131 2.2 3.7 1.0
NE2 A:HIS69 2.3 7.0 1.0
CE1 A:HIS69 3.2 7.8 1.0
CD2 A:HIS69 3.3 6.4 1.0
OE2 A:GLU60 4.2 7.0 1.0
O A:HOH2132 4.3 8.4 1.0
O A:GLY70 4.3 8.0 1.0
ND1 A:HIS69 4.4 7.0 1.0
CG A:HIS69 4.4 5.9 1.0

Reference:

L.M.Polo, F.Gil-Ortiz, A.Cantin, V.Rubio. New Insight Into the Transcarbamylase Family: the Structure of Putrescine Transcarbamylase, A Key Catalyst For Fermentative Utilization of Agmatine Plos One V. 7 31528 2012.
ISSN: ISSN 1932-6203
PubMed: 22363663
DOI: 10.1371/JOURNAL.PONE.0031528
Page generated: Wed Dec 16 01:29:08 2020

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