Nickel in PDB 4am8: Crystal Structure of the R54G Mutant of Putrescine Transcarbamylase From Enterococcus Faecalis Bound to A Curing Guanidinium Ion

All present enzymatic activity of Crystal Structure of the R54G Mutant of Putrescine Transcarbamylase From Enterococcus Faecalis Bound to A Curing Guanidinium Ion:
2.1.3.6;

The structure of Crystal Structure of the R54G Mutant of Putrescine Transcarbamylase From Enterococcus Faecalis Bound to A Curing Guanidinium Ion, PDB code: 4am8 was solved by L.M.Polo, V.Rubio, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	25.00 / 1.99
Space group	I 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	103.690, 130.600, 164.157, 90.00, 104.25, 90.00
R / Rfree (%)	18.2 / 20.6

The binding sites of Nickel atom in the Crystal Structure of the R54G Mutant of Putrescine Transcarbamylase From Enterococcus Faecalis Bound to A Curing Guanidinium Ion (pdb code 4am8). This binding sites where shown within 5.0 Angstroms radius around Nickel atom.
In total 2 binding sites of Nickel where determined in the Crystal Structure of the R54G Mutant of Putrescine Transcarbamylase From Enterococcus Faecalis Bound to A Curing Guanidinium Ion, PDB code: 4am8:
Jump to Nickel binding site number: 1; 2;

Nickel binding site 1 out of 2 in the Crystal Structure of the R54G Mutant of Putrescine Transcarbamylase From Enterococcus Faecalis Bound to A Curing Guanidinium Ion


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 1 of Crystal Structure of the R54G Mutant of Putrescine Transcarbamylase From Enterococcus Faecalis Bound to A Curing Guanidinium Ion within 5.0Å range: probe atom residue distance (Å) B Occ A:Ni405 b:15.4 occ:1.00 O B:HOH531 2.2 10.0 1.0 O C:HOH540 2.2 11.8 1.0 NE2 A:HIS69 2.2 14.4 1.0 O A:HOH563 2.2 10.2 1.0 NE2 B:HIS69 2.2 13.5 1.0 NE2 C:HIS69 2.3 15.1 1.0 CE1 A:HIS69 3.2 14.8 1.0 CE1 B:HIS69 3.2 13.5 1.0 CD2 A:HIS69 3.2 14.2 1.0 CD2 B:HIS69 3.2 13.3 1.0 CE1 C:HIS69 3.2 15.3 1.0 CD2 C:HIS69 3.3 14.8 1.0 OE2 B:GLU60 4.2 18.3 1.0 O B:HOH505 4.2 15.6 1.0 O B:GLY70 4.3 13.9 1.0 O A:HOH505 4.3 20.3 1.0 OE2 C:GLU60 4.3 17.5 1.0 O C:GLY70 4.3 14.7 1.0 O A:GLY70 4.4 14.6 1.0 ND1 A:HIS69 4.4 14.5 1.0 ND1 B:HIS69 4.4 13.4 1.0 OE2 A:GLU60 4.4 16.5 1.0 ND1 C:HIS69 4.4 14.8 1.0 CG A:HIS69 4.4 14.2 1.0 CG B:HIS69 4.4 13.2 1.0 O C:HOH551 4.4 18.3 1.0 CG C:HIS69 4.4 14.6 1.0

Nickel binding site 2 out of 2 in the Crystal Structure of the R54G Mutant of Putrescine Transcarbamylase From Enterococcus Faecalis Bound to A Curing Guanidinium Ion


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 2 of Crystal Structure of the R54G Mutant of Putrescine Transcarbamylase From Enterococcus Faecalis Bound to A Curing Guanidinium Ion within 5.0Å range: probe atom residue distance (Å) B Occ F:Ni401 b:19.9 occ:1.00 O F:HOH537 2.1 14.1 1.0 O E:HOH543 2.2 16.0 1.0 NE2 E:HIS69 2.2 16.8 1.0 NE2 D:HIS69 2.2 17.3 1.0 O D:HOH510 2.2 17.0 1.0 NE2 F:HIS69 2.3 16.2 1.0 CE1 D:HIS69 3.2 17.2 1.0 CE1 E:HIS69 3.2 17.4 1.0 CD2 E:HIS69 3.2 16.4 1.0 CD2 F:HIS69 3.2 16.2 1.0 CE1 F:HIS69 3.2 16.7 1.0 CD2 D:HIS69 3.2 16.6 1.0 OE2 F:GLU60 4.2 18.1 1.0 O D:GLY70 4.3 17.8 1.0 O F:GLY70 4.3 16.4 1.0 OE2 D:GLU60 4.3 22.3 1.0 ND1 D:HIS69 4.3 16.8 1.0 ND1 E:HIS69 4.4 17.1 1.0 OE2 E:GLU60 4.4 22.0 1.0 O E:GLY70 4.4 18.8 1.0 ND1 F:HIS69 4.4 16.4 1.0 CG E:HIS69 4.4 16.4 1.0 O E:HOH522 4.4 21.2 1.0 CG D:HIS69 4.4 16.4 1.0 CG F:HIS69 4.4 16.0 1.0 O F:HOH508 4.5 21.2 1.0 O D:HOH523 4.5 21.7 1.0

L.M.Polo, V.Rubio. Curing A Mutant Transcarbamylase with A Small Molecule: A Crystallographic View To Be Published.