Nickel in PDB 4j72: Crystal Structure of Polyprenyl-Phosphate N-Acetyl Hexosamine 1- Phosphate Transferase

All present enzymatic activity of Crystal Structure of Polyprenyl-Phosphate N-Acetyl Hexosamine 1- Phosphate Transferase:
2.7.8.13;

The structure of Crystal Structure of Polyprenyl-Phosphate N-Acetyl Hexosamine 1- Phosphate Transferase, PDB code: 4j72 was solved by S.Y.Lee, B.C.Chung, R.A.Gillespie, D.Y.Kwon, Z.Guan, P.Zhou, J.Hong, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	26.69 / 3.30
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	95.799, 101.491, 138.313, 90.00, 90.00, 90.00
R / Rfree (%)	22.3 / 26.5

The structure of Crystal Structure of Polyprenyl-Phosphate N-Acetyl Hexosamine 1- Phosphate Transferase also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

The binding sites of Nickel atom in the Crystal Structure of Polyprenyl-Phosphate N-Acetyl Hexosamine 1- Phosphate Transferase (pdb code 4j72). This binding sites where shown within 5.0 Angstroms radius around Nickel atom.
In total 2 binding sites of Nickel where determined in the Crystal Structure of Polyprenyl-Phosphate N-Acetyl Hexosamine 1- Phosphate Transferase, PDB code: 4j72:
Jump to Nickel binding site number: 1; 2;

Nickel binding site 1 out of 2 in the Crystal Structure of Polyprenyl-Phosphate N-Acetyl Hexosamine 1- Phosphate Transferase


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 1 of Crystal Structure of Polyprenyl-Phosphate N-Acetyl Hexosamine 1- Phosphate Transferase within 5.0Å range: probe atom residue distance (Å) B Occ A:Ni401 b:33.5 occ:1.00 NE2 A:HIS325 2.1 34.3 1.0 CE1 A:HIS324 2.1 32.4 1.0 NE2 A:HIS324 2.1 33.9 1.0 CE1 A:HIS325 2.8 35.9 1.0 CD2 A:HIS325 3.2 33.5 1.0 ND1 A:HIS324 3.4 31.4 1.0 CD2 A:HIS324 3.5 34.0 1.0 ND1 A:HIS325 4.0 36.2 1.0 CG A:HIS324 4.1 32.4 1.0 CG A:HIS325 4.2 34.7 1.0

Nickel binding site 2 out of 2 in the Crystal Structure of Polyprenyl-Phosphate N-Acetyl Hexosamine 1- Phosphate Transferase


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 2 of Crystal Structure of Polyprenyl-Phosphate N-Acetyl Hexosamine 1- Phosphate Transferase within 5.0Å range: probe atom residue distance (Å) B Occ A:Ni402 b:25.4 occ:1.00 OE2 A:GLU328 2.0 43.1 1.0 ND1 A:HIS326 2.2 33.1 1.0 CD A:GLU328 3.0 44.3 1.0 CE1 A:HIS326 3.1 32.0 1.0 CG A:HIS326 3.2 33.1 1.0 OE1 A:GLU328 3.4 45.5 1.0 CB A:HIS326 3.6 34.4 1.0 OE1 A:GLU334 3.6 47.8 1.0 CG A:GLU328 4.2 44.6 1.0 NE2 A:HIS326 4.2 31.4 1.0 CD2 A:HIS326 4.3 32.0 1.0 CD A:GLU334 4.7 47.2 1.0 CA A:HIS326 4.8 35.6 1.0 O A:LEU327 4.8 40.1 1.0

B.C.Chung, J.Zhao, R.A.Gillespie, D.Y.Kwon, Z.Guan, J.Hong, P.Zhou, S.Y.Lee. Crystal Structure of Mray, An Essential Membrane Enzyme For Bacterial Cell Wall Synthesis. Science V. 341 1012 2013.
ISSN: ISSN 0036-8075
PubMed: 23990562
DOI: 10.1126/SCIENCE.1236501