Nickel in PDB 4ra0: An Engineered Axl 'Decoy Receptor' Effectively Silences the GAS6-Axl Signaling Axis

All present enzymatic activity of An Engineered Axl 'Decoy Receptor' Effectively Silences the GAS6-Axl Signaling Axis:
2.7.10.1;

The structure of An Engineered Axl 'Decoy Receptor' Effectively Silences the GAS6-Axl Signaling Axis, PDB code: 4ra0 was solved by M.S.Kariolis, S.Kapur, I.I.Mathews, J.R.Cochran, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	38.01 / 3.07
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	112.370, 112.370, 361.260, 90.00, 90.00, 120.00
R / Rfree (%)	20.2 / 24.3

The structure of An Engineered Axl 'Decoy Receptor' Effectively Silences the GAS6-Axl Signaling Axis also contains other interesting chemical elements:

Chlorine	(Cl)	1 atom
Calcium	(Ca)	2 atoms

The binding sites of Nickel atom in the An Engineered Axl 'Decoy Receptor' Effectively Silences the GAS6-Axl Signaling Axis (pdb code 4ra0). This binding sites where shown within 5.0 Angstroms radius around Nickel atom.
In total 2 binding sites of Nickel where determined in the An Engineered Axl 'Decoy Receptor' Effectively Silences the GAS6-Axl Signaling Axis, PDB code: 4ra0:
Jump to Nickel binding site number: 1; 2;

Nickel binding site 1 out of 2 in the An Engineered Axl 'Decoy Receptor' Effectively Silences the GAS6-Axl Signaling Axis


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 1 of An Engineered Axl 'Decoy Receptor' Effectively Silences the GAS6-Axl Signaling Axis within 5.0Å range: probe atom residue distance (Å) B Occ C:Ni301 b:0.8 occ:1.00 ND1 C:HIS179 2.0 0.1 1.0 CE1 C:HIS179 2.1 0.1 1.0 CG C:HIS179 2.8 0.1 1.0 NE2 C:HIS179 2.9 0.1 1.0 CD2 C:HIS179 3.2 0.4 1.0 CB C:HIS179 3.8 0.6 1.0 O C:PRO177 4.7 0.8 1.0 CB C:ALA176 4.8 0.1 1.0 N C:HIS179 5.0 0.0 1.0

Nickel binding site 2 out of 2 in the An Engineered Axl 'Decoy Receptor' Effectively Silences the GAS6-Axl Signaling Axis


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 2 of An Engineered Axl 'Decoy Receptor' Effectively Silences the GAS6-Axl Signaling Axis within 5.0Å range: probe atom residue distance (Å) B Occ D:Ni301 b:0.9 occ:1.00 NE2 D:HIS179 2.0 0.2 1.0 CE1 D:HIS179 2.4 0.1 1.0 CD2 D:HIS179 3.4 0.9 1.0 ND1 D:HIS179 3.7 0.3 1.0 CG D:HIS179 4.2 0.3 1.0

M.S.Kariolis, Y.R.Miao, D.S.Jones, S.Kapur, I.I.Mathews, A.J.Giaccia, J.R.Cochran. An Engineered Axl 'Decoy Receptor' Effectively Silences the GAS6-Axl Signaling Axis. Nat.Chem.Biol. V. 10 977 2014.
ISSN: ISSN 1552-4450
PubMed: 25242553
DOI: 10.1038/NCHEMBIO.1636