Nickel in PDB 8c6i: TMEM2 Ectodomain

All present enzymatic activity of TMEM2 Ectodomain:
3.2.1.35;

The structure of TMEM2 Ectodomain, PDB code: 8c6i was solved by E.Hohenester, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	91.55 / 3.50
Space group	P 42 21 2
Cell size a, b, c (Å), α, β, γ (°)	184.76, 184.76, 105.398, 90, 90, 90
R / Rfree (%)	26.5 / 31.9

The structure of TMEM2 Ectodomain also contains other interesting chemical elements:

Chlorine

(Cl)

1 atom

The binding sites of Nickel atom in the TMEM2 Ectodomain (pdb code 8c6i). This binding sites where shown within 5.0 Angstroms radius around Nickel atom.
In total only one binding site of Nickel was determined in the TMEM2 Ectodomain, PDB code: 8c6i:

Nickel binding site 1 out of 1 in the TMEM2 Ectodomain


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 1 of TMEM2 Ectodomain within 5.0Å range: probe atom residue distance (Å) B Occ A:Ni9901 b:111.9 occ:1.00 NE2 A:HIS550 1.9 110.7 1.0 ND1 A:HIS585 2.1 108.4 1.0 ND1 A:HIS552 2.2 111.5 1.0 CE1 A:HIS550 2.4 110.7 1.0 CG A:HIS552 2.6 111.5 1.0 CG A:HIS585 2.9 108.4 1.0 CE1 A:HIS552 2.9 111.5 1.0 CB A:HIS552 3.0 111.5 1.0 CE1 A:HIS585 3.0 108.4 1.0 CD2 A:HIS550 3.1 110.7 1.0 CB A:HIS585 3.2 108.4 1.0 CD2 A:HIS552 3.4 111.5 1.0 CA A:HIS552 3.5 111.5 1.0 ND1 A:HIS550 3.6 110.7 1.0 NE2 A:HIS552 3.6 111.5 1.0 CG A:HIS550 3.9 110.7 1.0 CD2 A:HIS585 4.0 108.4 1.0 NE2 A:HIS585 4.0 108.4 1.0 N A:HIS552 4.4 111.5 1.0 OE2 A:GLU608 4.5 106.7 1.0 C A:HIS552 4.5 111.5 1.0 O A:PHE551 4.6 111.8 1.0 CA A:HIS585 4.7 108.4 1.0 C A:PHE551 4.8 111.8 1.0 N A:LEU553 4.9 109.3 1.0

M.Niu, M.Mcgrath, D.Sammon, S.Gardner, R.M.Morgan, D.Bubeck, E.Hohenester. Structure of the TMEM2 Ectodomain and Its Lack of Hyaluronidase Activity To Be Published.