Nickel in PDB 8qou: Reactive Intermediate Deaminase A Mutant - R107K

All present enzymatic activity of Reactive Intermediate Deaminase A Mutant - R107K:
3.5.99.10;

The structure of Reactive Intermediate Deaminase A Mutant - R107K, PDB code: 8qou was solved by G.Rizzi, C.Visentin, F.Di Pisa, S.Ricagno, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.50 / 1.40
Space group	P 21 3
Cell size a, b, c (Å), α, β, γ (°)	90.482, 90.482, 90.482, 90, 90, 90
R / Rfree (%)	14.9 / 17

The binding sites of Nickel atom in the Reactive Intermediate Deaminase A Mutant - R107K (pdb code 8qou). This binding sites where shown within 5.0 Angstroms radius around Nickel atom.
In total 2 binding sites of Nickel where determined in the Reactive Intermediate Deaminase A Mutant - R107K, PDB code: 8qou:
Jump to Nickel binding site number: 1; 2;

Nickel binding site 1 out of 2 in the Reactive Intermediate Deaminase A Mutant - R107K


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 1 of Reactive Intermediate Deaminase A Mutant - R107K within 5.0Å range: probe atom residue distance (Å) B Occ A:Ni203 b:14.0 occ:1.00 N A:SER-1 1.9 14.0 1.0 N A:HIS0 2.0 13.7 1.0 ND1 A:HIS0 2.0 13.8 1.0 N A:GLY-2 2.0 14.4 1.0 C A:SER-1 2.8 15.2 1.0 C A:GLY-2 2.8 14.8 1.0 CA A:SER-1 2.9 14.8 1.0 CA A:GLY-2 2.9 15.1 1.0 CE1 A:HIS0 3.0 13.2 1.0 CG A:HIS0 3.0 13.0 1.0 CA A:HIS0 3.1 13.0 1.0 CB A:HIS0 3.3 13.7 1.0 N A:MET1 3.7 12.6 1.0 OG A:SER-1 3.7 19.3 1.0 C A:HIS0 3.8 14.2 1.0 CB A:SER-1 3.8 15.7 1.0 O A:SER-1 4.0 15.6 1.0 O A:GLY-2 4.0 15.9 1.0 NE2 A:HIS0 4.1 14.2 1.0 CD2 A:HIS0 4.1 13.8 1.0 O A:HOH408 4.3 16.9 1.0 CA A:MET1 4.9 11.8 1.0 O A:HIS0 5.0 16.0 1.0 CB A:MET1 5.0 15.5 1.0

Nickel binding site 2 out of 2 in the Reactive Intermediate Deaminase A Mutant - R107K


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 2 of Reactive Intermediate Deaminase A Mutant - R107K within 5.0Å range: probe atom residue distance (Å) B Occ B:Ni401 b:14.5 occ:1.00 N B:SER-1 1.9 16.6 1.0 ND1 B:HIS0 1.9 15.3 1.0 N B:HIS0 2.0 12.2 1.0 N B:GLY-2 2.0 19.6 1.0 C B:GLY-2 2.8 19.7 1.0 C B:SER-1 2.8 13.8 1.0 CA B:SER-1 2.9 16.2 1.0 CA B:GLY-2 2.9 19.4 1.0 CG B:HIS0 2.9 14.4 1.0 CE1 B:HIS0 3.0 15.4 1.0 CA B:HIS0 3.0 11.9 1.0 CB B:HIS0 3.2 12.9 1.0 N B:MET1 3.6 9.8 1.0 C B:HIS0 3.7 9.8 1.0 OG B:SER-1 3.8 19.8 1.0 CB B:SER-1 3.9 16.8 1.0 O B:GLY-2 4.0 22.4 1.0 O B:SER-1 4.0 14.8 1.0 CD2 B:HIS0 4.0 15.2 1.0 NE2 B:HIS0 4.1 16.4 1.0 O B:HOH605 4.4 21.6 1.0 CA B:MET1 4.8 9.8 1.0 O B:HIS0 4.8 9.8 1.0 O B:MET1 5.0 9.6 1.0

G.Rizzi, C.Visentin, F.Di Pisa, S.Ricagno. Reactive Intermediate Deaminase A Mutant - R107K To Be Published.