Nickel in PDB 1s6m: Conjugative Relaxase Trwc in Complex with Orit Dna. Metal- Bound Structure

The structure of Conjugative Relaxase Trwc in Complex with Orit Dna. Metal- Bound Structure, PDB code: 1s6m was solved by A.Guasch, M.Lucas, G.Moncalian, M.Cabezas, R.Perez-Luque, F.X.Gomis-Ruth, F.De La Cruz, M.Coll, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.85 / 2.28
Space group	P 61 2 2
Cell size a, b, c (Å), α, β, γ (°)	92.731, 92.731, 208.855, 90.00, 90.00, 120.00
R / Rfree (%)	20.6 / 24.5

The binding sites of Nickel atom in the Conjugative Relaxase Trwc in Complex with Orit Dna. Metal- Bound Structure (pdb code 1s6m). This binding sites where shown within 5.0 Angstroms radius around Nickel atom.
In total only one binding site of Nickel was determined in the Conjugative Relaxase Trwc in Complex with Orit Dna. Metal- Bound Structure, PDB code: 1s6m:

Nickel binding site 1 out of 1 in the Conjugative Relaxase Trwc in Complex with Orit Dna. Metal- Bound Structure


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 1 of Conjugative Relaxase Trwc in Complex with Orit Dna. Metal- Bound Structure within 5.0Å range: probe atom residue distance (Å) B Occ A:Ni500 b:17.8 occ:1.00 ND1 A:HIS150 2.1 22.7 1.0 NE2 A:HIS161 2.2 17.5 1.0 O A:HOH501 2.3 30.5 1.0 NE2 A:HIS163 2.3 26.3 1.0 O A:HOH502 2.3 34.9 1.0 CE1 A:HIS150 3.1 23.1 1.0 CE1 A:HIS161 3.1 18.0 1.0 CD2 A:HIS161 3.2 17.8 1.0 CG A:HIS150 3.2 21.9 1.0 CD2 A:HIS163 3.2 22.6 1.0 CE1 A:HIS163 3.3 25.0 1.0 CB A:HIS150 3.5 20.3 1.0 O A:HOH620 4.2 49.6 1.0 NE2 A:HIS150 4.3 21.4 1.0 ND1 A:HIS161 4.3 19.7 1.0 CD2 A:HIS150 4.3 22.5 1.0 CG A:HIS161 4.3 18.3 1.0 ND1 A:HIS163 4.4 24.9 1.0 CG A:HIS163 4.4 22.4 1.0 OD1 A:ASP21 4.5 65.5 1.0 CB A:ASP21 4.6 64.5 1.0

R.Boer, S.Russi, A.Guasch, M.Lucas, A.G.Blanco, R.Perez-Luque, M.Coll, F.De La Cruz. Unveiling the Molecular Mechanism of A Conjugative Relaxase: the Structure of Trwc Complexed with A 27-Mer Dna Comprising the Recognition Hairpin and the Cleavage Site. J.Mol.Biol. V. 358 857 2006.
ISSN: ISSN 0022-2836
PubMed: 16540117
DOI: 10.1016/J.JMB.2006.02.018