Nickel in PDB 5lne: E. Coli F9 Pilus Adhesin Fmlh Bound to the Thomsen-Friedenreich (Tf) Antigen

The structure of E. Coli F9 Pilus Adhesin Fmlh Bound to the Thomsen-Friedenreich (Tf) Antigen, PDB code: 5lne was solved by S.Ruer, M.S.Conover, V.Kalas, J.Taganna, H.De Greve, J.S.Pinkner, K.W.Dodson, S.J.Hultgren, H.Remaut, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	59.40 / 2.20
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	118.820, 51.460, 53.740, 90.00, 90.00, 90.00
R / Rfree (%)	18 / 22.4

The binding sites of Nickel atom in the E. Coli F9 Pilus Adhesin Fmlh Bound to the Thomsen-Friedenreich (Tf) Antigen (pdb code 5lne). This binding sites where shown within 5.0 Angstroms radius around Nickel atom.
In total only one binding site of Nickel was determined in the E. Coli F9 Pilus Adhesin Fmlh Bound to the Thomsen-Friedenreich (Tf) Antigen, PDB code: 5lne:

Nickel binding site 1 out of 1 in the E. Coli F9 Pilus Adhesin Fmlh Bound to the Thomsen-Friedenreich (Tf) Antigen


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 1 of E. Coli F9 Pilus Adhesin Fmlh Bound to the Thomsen-Friedenreich (Tf) Antigen within 5.0Å range: probe atom residue distance (Å) B Occ B:Ni203 b:75.2 occ:1.00 ND1 B:HIS161 3.4 42.7 1.0 N B:HIS160 3.7 44.1 1.0 CB B:HIS164 4.0 57.9 1.0 ND1 B:HIS160 4.1 46.5 1.0 CE1 B:HIS161 4.2 40.4 1.0 N B:HIS161 4.3 43.1 1.0 ND1 B:HIS159 4.4 61.7 1.0 CA B:HIS159 4.4 51.9 1.0 CG B:HIS161 4.4 41.2 1.0 CA B:HIS160 4.5 44.0 1.0 CB B:HIS160 4.5 43.6 1.0 CB B:HIS159 4.5 54.1 1.0 CB B:HIS161 4.6 43.0 1.0 C B:HIS159 4.6 48.9 1.0 C B:HIS160 4.7 41.4 1.0 CG B:HIS160 4.8 47.9 1.0 CA B:HIS164 4.9 56.1 1.0 CG B:HIS159 4.9 57.8 1.0

M.S.Conover, S.Ruer, J.Taganna, V.Kalas, H.De Greve, J.S.Pinkner, K.W.Dodson, H.Remaut, S.J.Hultgren. Inflammation-Induced Adhesin-Receptor Interaction Provides A Fitness Advantage to Uropathogenic E. Coli During Chronic Infection. Cell Host Microbe V. 20 482 2016.
ISSN: ESSN 1934-6069
PubMed: 27667696
DOI: 10.1016/J.CHOM.2016.08.013