Nickel in PDB 3a3v: Crystal Structure of Reducing-End-Xylose Releasing Exo-Oligoxylanase Y198F Mutant

All present enzymatic activity of Crystal Structure of Reducing-End-Xylose Releasing Exo-Oligoxylanase Y198F Mutant:
3.2.1.156;

The structure of Crystal Structure of Reducing-End-Xylose Releasing Exo-Oligoxylanase Y198F Mutant, PDB code: 3a3v was solved by M.Hidaka, S.Fushinobu, Y.Honda, M.Kitaoka, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.50 / 1.39
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	53.191, 86.207, 87.729, 90.00, 90.00, 90.00
R / Rfree (%)	17.7 / 19.8

The binding sites of Nickel atom in the Crystal Structure of Reducing-End-Xylose Releasing Exo-Oligoxylanase Y198F Mutant (pdb code 3a3v). This binding sites where shown within 5.0 Angstroms radius around Nickel atom.
In total only one binding site of Nickel was determined in the Crystal Structure of Reducing-End-Xylose Releasing Exo-Oligoxylanase Y198F Mutant, PDB code: 3a3v:

Nickel binding site 1 out of 1 in the Crystal Structure of Reducing-End-Xylose Releasing Exo-Oligoxylanase Y198F Mutant


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 1 of Crystal Structure of Reducing-End-Xylose Releasing Exo-Oligoxylanase Y198F Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Ni1400 b:8.8 occ:1.00 OE1 A:GLU30 2.0 10.6 1.0 OE2 A:GLU27 2.1 11.9 1.0 OE1 A:GLU27 2.7 10.4 1.0 CD A:GLU27 2.7 9.8 1.0 CD A:GLU30 2.9 10.1 1.0 OE2 A:GLU30 3.1 12.3 1.0 CG A:GLU27 4.2 8.9 1.0 O A:HOH551 4.2 10.2 1.0 CG A:GLU30 4.3 10.5 1.0 O A:HOH575 4.5 13.2 1.0 CB A:GLU30 4.6 9.9 1.0 CA A:GLU27 4.9 10.0 1.0

M.Hidaka, S.Fushinobu, Y.Honda, T.Wakagi, H.Shoun, M.Kitaoka. Structural Explanation For the Acquisition of Glycosynthase Activity J.Biochem. V. 147 237 2010.
ISSN: ISSN 0021-924X
PubMed: 19819900
DOI: 10.1093/JB/MVP159