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Nickel in PDB 4zf6: Cytochrome P450 Pentamutant From BM3 with Bound Peg

Enzymatic activity of Cytochrome P450 Pentamutant From BM3 with Bound Peg

All present enzymatic activity of Cytochrome P450 Pentamutant From BM3 with Bound Peg:
1.14.14.1; 1.6.2.4;

Protein crystallography data

The structure of Cytochrome P450 Pentamutant From BM3 with Bound Peg, PDB code: 4zf6 was solved by W.E.Rogers, T.Othman, D.K.Heidary, T.Huxford, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 59.27 / 2.77
Space group P 65 2 2
Cell size a, b, c (Å), α, β, γ (°) 56.047, 56.047, 711.229, 90.00, 90.00, 120.00
R / Rfree (%) 20.1 / 25.7

Other elements in 4zf6:

The structure of Cytochrome P450 Pentamutant From BM3 with Bound Peg also contains other interesting chemical elements:

Iron (Fe) 1 atom

Nickel Binding Sites:

The binding sites of Nickel atom in the Cytochrome P450 Pentamutant From BM3 with Bound Peg (pdb code 4zf6). This binding sites where shown within 5.0 Angstroms radius around Nickel atom.
In total 4 binding sites of Nickel where determined in the Cytochrome P450 Pentamutant From BM3 with Bound Peg, PDB code: 4zf6:
Jump to Nickel binding site number: 1; 2; 3; 4;

Nickel binding site 1 out of 4 in 4zf6

Go back to Nickel Binding Sites List in 4zf6
Nickel binding site 1 out of 4 in the Cytochrome P450 Pentamutant From BM3 with Bound Peg


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 1 of Cytochrome P450 Pentamutant From BM3 with Bound Peg within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ni504

b:75.9
occ:1.00
NE2 A:HIS138 2.0 86.1 1.0
NE2 A:HIS426 2.0 81.3 1.0
CE1 A:HIS138 2.7 80.3 1.0
CD2 A:HIS426 2.9 68.0 1.0
CE1 A:HIS426 3.0 78.2 1.0
CD2 A:HIS138 3.2 74.4 1.0
NZ A:LYS447 3.9 59.9 1.0
ND1 A:HIS138 3.9 53.4 1.0
CG A:HIS426 4.1 68.5 1.0
ND1 A:HIS426 4.1 62.5 1.0
CG A:HIS138 4.2 61.0 1.0
O A:ASP136 4.7 65.0 1.0

Nickel binding site 2 out of 4 in 4zf6

Go back to Nickel Binding Sites List in 4zf6
Nickel binding site 2 out of 4 in the Cytochrome P450 Pentamutant From BM3 with Bound Peg


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 2 of Cytochrome P450 Pentamutant From BM3 with Bound Peg within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ni505

b:86.5
occ:1.00
NE2 A:HIS285 2.0 87.0 1.0
CD2 A:HIS285 3.0 73.2 1.0
CE1 A:HIS285 3.0 68.0 1.0
ND1 A:HIS285 4.1 52.5 1.0
CG A:HIS285 4.1 56.9 1.0
OE1 A:GLN288 4.7 46.3 1.0

Nickel binding site 3 out of 4 in 4zf6

Go back to Nickel Binding Sites List in 4zf6
Nickel binding site 3 out of 4 in the Cytochrome P450 Pentamutant From BM3 with Bound Peg


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 3 of Cytochrome P450 Pentamutant From BM3 with Bound Peg within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ni506

b:64.9
occ:1.00
OG1 A:THR1 2.0 83.9 1.0
N A:THR1 2.0 39.1 1.0
CA A:THR1 2.3 48.1 1.0
CB A:THR1 2.6 66.1 1.0
CG2 A:THR1 3.6 79.0 1.0
C A:THR1 3.8 69.6 1.0
O A:THR1 4.4 77.2 1.0
O A:GLY343 4.5 69.9 1.0
N A:ILE2 4.7 63.0 1.0

Nickel binding site 4 out of 4 in 4zf6

Go back to Nickel Binding Sites List in 4zf6
Nickel binding site 4 out of 4 in the Cytochrome P450 Pentamutant From BM3 with Bound Peg


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 4 of Cytochrome P450 Pentamutant From BM3 with Bound Peg within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ni507

b:0.3
occ:1.00
CE1 A:HIS236 0.9 0.0 1.0
NE2 A:HIS236 2.0 98.6 1.0
OD1 A:ASP231 2.0 96.9 1.0
ND1 A:HIS236 2.0 80.8 1.0
OD2 A:ASP231 2.0 0.4 1.0
CG A:ASP231 2.2 0.5 1.0
CD2 A:HIS236 3.0 93.9 1.0
CG A:HIS236 3.1 54.9 1.0
CB A:ASP231 3.7 83.2 1.0
O A:ASP231 4.3 89.8 1.0
CA A:ASP231 4.3 81.2 1.0
CB A:HIS236 4.4 44.9 1.0
C A:ASP231 4.5 75.3 1.0

Reference:

I.Geronimo, C.A.Denning, W.E.Rogers, T.Othman, T.Huxford, D.K.Heidary, E.C.Glazer, C.M.Payne. Effect of Mutation and Substrate Binding on the Stability of Cytochrome P450BM3 Variants. Biochemistry V. 55 3594 2016.
ISSN: ISSN 0006-2960
PubMed: 27267136
DOI: 10.1021/ACS.BIOCHEM.6B00183
Page generated: Mon Aug 18 19:51:50 2025

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