Nickel in PDB 5ltw: Complex of Human 14-3-3 Sigma CLU1 Mutant with Phosphorylated Heat Shock Protein B6

The structure of Complex of Human 14-3-3 Sigma CLU1 Mutant with Phosphorylated Heat Shock Protein B6, PDB code: 5ltw was solved by N.N.Sluchanko, S.Beelen, A.A.Kulikova, S.D.Weeks, A.A.Antson, N.B.Gusev, S.V.Strelkov, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.17 / 4.50
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	125.060, 341.290, 144.550, 90.00, 90.00, 90.00
R / Rfree (%)	23.6 / 25.6

The binding sites of Nickel atom in the Complex of Human 14-3-3 Sigma CLU1 Mutant with Phosphorylated Heat Shock Protein B6 (pdb code 5ltw). This binding sites where shown within 5.0 Angstroms radius around Nickel atom.
In total 3 binding sites of Nickel where determined in the Complex of Human 14-3-3 Sigma CLU1 Mutant with Phosphorylated Heat Shock Protein B6, PDB code: 5ltw:
Jump to Nickel binding site number: 1; 2; 3;

Nickel binding site 1 out of 3 in the Complex of Human 14-3-3 Sigma CLU1 Mutant with Phosphorylated Heat Shock Protein B6


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 1 of Complex of Human 14-3-3 Sigma CLU1 Mutant with Phosphorylated Heat Shock Protein B6 within 5.0Å range: probe atom residue distance (Å) B Occ D:Ni201 b:60.0 occ:1.00 CA D:GLY140 3.4 75.5 1.0 O D:PHE83 3.8 69.7 1.0 N D:GLY140 4.1 77.2 1.0 O D:LYS81 4.3 72.0 1.0 O D:VAL80 4.4 71.0 1.0 C D:GLY140 4.6 76.3 1.0 CA D:LYS81 4.6 72.7 1.0 CD2 D:LEU136 4.7 71.8 1.0 O D:SER137 4.7 77.2 1.0 C D:LYS81 4.8 71.9 1.0 C D:PHE83 4.9 69.4 1.0 C D:GLU139 4.9 78.7 1.0 O D:PRO138 4.9 78.6 1.0 CG D:LEU136 4.9 73.5 1.0 CD1 D:LEU136 4.9 73.9 1.0 O D:GLY140 5.0 75.6 1.0

Nickel binding site 2 out of 3 in the Complex of Human 14-3-3 Sigma CLU1 Mutant with Phosphorylated Heat Shock Protein B6


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 2 of Complex of Human 14-3-3 Sigma CLU1 Mutant with Phosphorylated Heat Shock Protein B6 within 5.0Å range: probe atom residue distance (Å) B Occ H:Ni201 b:60.0 occ:1.00 CA H:GLY140 3.6 70.1 1.0 O H:PHE83 4.0 62.9 1.0 O H:LYS81 4.1 62.4 1.0 N H:GLY140 4.1 73.8 1.0 O H:PRO138 4.6 81.3 1.0 CA H:LYS81 4.6 61.7 1.0 O H:VAL80 4.7 60.0 1.0 C H:LYS81 4.7 60.6 1.0 C H:GLU139 4.8 75.5 1.0 C H:GLY140 4.9 69.1 1.0 O H:SER137 4.9 77.3 1.0 C H:PHE83 5.0 61.7 1.0

Nickel binding site 3 out of 3 in the Complex of Human 14-3-3 Sigma CLU1 Mutant with Phosphorylated Heat Shock Protein B6


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 3 of Complex of Human 14-3-3 Sigma CLU1 Mutant with Phosphorylated Heat Shock Protein B6 within 5.0Å range: probe atom residue distance (Å) B Occ L:Ni201 b:60.0 occ:1.00 O L:PRO138 2.6 68.8 1.0 C L:PRO138 3.1 68.3 1.0 N L:GLY140 3.3 64.9 1.0 O L:SER137 3.6 63.8 1.0 CA L:PRO138 3.6 67.6 1.0 CA L:GLY140 3.7 62.9 1.0 C L:GLU139 3.8 67.1 1.0 N L:GLU139 3.8 68.7 1.0 CA L:GLU139 4.2 69.5 1.0 C L:SER137 4.5 65.1 1.0 O L:GLU139 4.5 67.5 1.0 N L:PRO138 4.6 67.0 1.0 CB L:PRO138 4.8 70.0 1.0

N.N.Sluchanko, S.Beelen, A.A.Kulikova, S.D.Weeks, A.A.Antson, N.B.Gusev, S.V.Strelkov. Structural Basis For the Interaction of A Human Small Heat Shock Protein with the 14-3-3 Universal Signaling Regulator. Structure V. 25 305 2017.
ISSN: ISSN 1878-4186
PubMed: 28089448
DOI: 10.1016/J.STR.2016.12.005