Nickel in PDB 5o7y: Thebaine 6-O-Demethylase (T6ODM) From Papaver Somniferum in Complex with Succinate

All present enzymatic activity of Thebaine 6-O-Demethylase (T6ODM) From Papaver Somniferum in Complex with Succinate:
1.14.11.31;

The structure of Thebaine 6-O-Demethylase (T6ODM) From Papaver Somniferum in Complex with Succinate, PDB code: 5o7y was solved by A.Kluza, E.Niedzialkowska, K.Kurpiewska, P.J.Porebski, T.Borowski, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	41.51 / 1.97
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	47.231, 87.028, 94.171, 90.00, 90.00, 90.00
R / Rfree (%)	16.3 / 20.7

The binding sites of Nickel atom in the Thebaine 6-O-Demethylase (T6ODM) From Papaver Somniferum in Complex with Succinate (pdb code 5o7y). This binding sites where shown within 5.0 Angstroms radius around Nickel atom.
In total only one binding site of Nickel was determined in the Thebaine 6-O-Demethylase (T6ODM) From Papaver Somniferum in Complex with Succinate, PDB code: 5o7y:

Nickel binding site 1 out of 1 in the Thebaine 6-O-Demethylase (T6ODM) From Papaver Somniferum in Complex with Succinate


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 1 of Thebaine 6-O-Demethylase (T6ODM) From Papaver Somniferum in Complex with Succinate within 5.0Å range: probe atom residue distance (Å) B Occ A:Ni401 b:18.6 occ:1.00 O1 A:EDO403 2.0 21.8 1.0 O2 A:EDO418 2.0 22.6 1.0 NE2 A:HIS295 2.1 17.7 1.0 O3 A:SIN402 2.1 18.6 1.0 NE2 A:HIS238 2.1 19.0 1.0 OD1 A:ASP240 2.1 18.4 1.0 C2 A:EDO418 2.9 27.4 1.0 C4 A:SIN402 3.0 19.1 1.0 CD2 A:HIS295 3.0 18.1 1.0 CE1 A:HIS238 3.1 20.4 1.0 CG A:ASP240 3.1 19.2 1.0 CE1 A:HIS295 3.1 18.6 1.0 CD2 A:HIS238 3.1 18.9 1.0 C1 A:EDO403 3.1 26.2 1.0 O4 A:SIN402 3.3 20.3 1.0 OD2 A:ASP240 3.3 19.7 1.0 O2 A:EDO403 3.7 26.4 1.0 C2 A:EDO403 4.0 26.9 1.0 C1 A:EDO418 4.2 28.0 1.0 ND1 A:HIS238 4.2 19.5 1.0 ND1 A:HIS295 4.2 18.5 1.0 CG A:HIS295 4.2 18.4 1.0 CG A:HIS238 4.3 20.1 1.0 C3 A:SIN402 4.3 18.9 1.0 CB A:ASP240 4.5 18.8 1.0 CD2 A:LEU235 4.7 19.7 1.0 N A:ASP240 4.8 18.9 1.0 CA A:ASP240 4.9 18.9 1.0 CE1 A:PHE311 4.9 18.5 1.0

A.Kluza, E.Niedzialkowska, K.Kurpiewska, Z.Wojdyla, M.Quesne, E.Kot, P.J.Porebski, T.Borowski. Crystal Structure of Thebaine 6-O-Demethylase From the Morphine Biosynthesis Pathway. J. Struct. Biol. V. 202 229 2018.
ISSN: ESSN 1095-8657
PubMed: 29408320
DOI: 10.1016/J.JSB.2018.01.007