Atomistry » Nickel » PDB 5xkt-6c8z » 5y34
Atomistry »
  Nickel »
    PDB 5xkt-6c8z »
      5y34 »

Nickel in PDB 5y34: Membrane-Bound Respiratory [Nife]-Hydrogenase From Hydrogenovibrio Marinus in A Ferricyanide-Oxidized Condition

Enzymatic activity of Membrane-Bound Respiratory [Nife]-Hydrogenase From Hydrogenovibrio Marinus in A Ferricyanide-Oxidized Condition

All present enzymatic activity of Membrane-Bound Respiratory [Nife]-Hydrogenase From Hydrogenovibrio Marinus in A Ferricyanide-Oxidized Condition:
1.12.5.1;

Protein crystallography data

The structure of Membrane-Bound Respiratory [Nife]-Hydrogenase From Hydrogenovibrio Marinus in A Ferricyanide-Oxidized Condition, PDB code: 5y34 was solved by Y.Shomura, K.S.Yoon, H.Nishihara, Y.Higuchi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.89 / 1.32
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 75.628, 116.943, 113.337, 90.00, 91.42, 90.00
R / Rfree (%) 13.1 / 15.8

Other elements in 5y34:

The structure of Membrane-Bound Respiratory [Nife]-Hydrogenase From Hydrogenovibrio Marinus in A Ferricyanide-Oxidized Condition also contains other interesting chemical elements:

Magnesium (Mg) 2 atoms
Iron (Fe) 26 atoms

Nickel Binding Sites:

The binding sites of Nickel atom in the Membrane-Bound Respiratory [Nife]-Hydrogenase From Hydrogenovibrio Marinus in A Ferricyanide-Oxidized Condition (pdb code 5y34). This binding sites where shown within 5.0 Angstroms radius around Nickel atom.
In total 2 binding sites of Nickel where determined in the Membrane-Bound Respiratory [Nife]-Hydrogenase From Hydrogenovibrio Marinus in A Ferricyanide-Oxidized Condition, PDB code: 5y34:
Jump to Nickel binding site number: 1; 2;

Nickel binding site 1 out of 2 in 5y34

Go back to Nickel Binding Sites List in 5y34
Nickel binding site 1 out of 2 in the Membrane-Bound Respiratory [Nife]-Hydrogenase From Hydrogenovibrio Marinus in A Ferricyanide-Oxidized Condition


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 1 of Membrane-Bound Respiratory [Nife]-Hydrogenase From Hydrogenovibrio Marinus in A Ferricyanide-Oxidized Condition within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ni601

b:15.4
occ:1.00
O A:O603 1.7 14.5 1.0
SG A:CYS590 2.1 16.8 1.0
SG A:CYS76 2.2 15.5 1.0
SG A:CYS79 2.4 11.8 1.0
SG A:CYS593 2.6 11.7 1.0
FE A:FCO602 2.8 11.0 1.0
CB A:CYS76 3.1 13.5 1.0
CB A:CYS590 3.2 14.4 1.0
CB A:CYS79 3.5 12.7 1.0
N A:CYS79 3.6 12.1 1.0
CB A:CYS593 3.7 11.6 1.0
C2 A:FCO602 3.9 12.1 1.0
C1 A:FCO602 4.0 11.9 1.0
CA A:CYS79 4.1 12.6 1.0
NH1 A:ARG523 4.2 12.4 1.0
CB A:VAL78 4.4 13.3 1.0
CA A:CYS76 4.5 10.9 1.0
C3 A:FCO602 4.5 11.2 1.0
CZ A:ARG523 4.5 9.8 1.0
CA A:CYS590 4.6 11.1 1.0
N A:CYS593 4.6 9.9 1.0
NE A:ARG523 4.7 10.1 1.0
CA A:CYS593 4.7 10.0 1.0
C A:VAL78 4.7 11.6 1.0
CD A:ARG523 4.8 10.2 1.0
N2 A:FCO602 4.8 10.5 1.0
N A:VAL78 4.9 12.4 1.0
CA A:VAL78 4.9 11.8 1.0
N1 A:FCO602 4.9 11.1 1.0

Nickel binding site 2 out of 2 in 5y34

Go back to Nickel Binding Sites List in 5y34
Nickel binding site 2 out of 2 in the Membrane-Bound Respiratory [Nife]-Hydrogenase From Hydrogenovibrio Marinus in A Ferricyanide-Oxidized Condition


Mono view


Stereo pair view

A full contact list of Nickel with other atoms in the Ni binding site number 2 of Membrane-Bound Respiratory [Nife]-Hydrogenase From Hydrogenovibrio Marinus in A Ferricyanide-Oxidized Condition within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Ni702

b:17.4
occ:1.00
O C:O704 1.7 16.3 1.0
SG C:CYS590 2.1 19.1 1.0
SG C:CYS76 2.2 16.9 1.0
SG C:CYS79 2.5 13.4 1.0
SG C:CYS593 2.6 13.2 1.0
FE C:FCO703 2.8 12.7 1.0
CB C:CYS76 3.1 16.2 1.0
CB C:CYS590 3.2 15.6 1.0
CB C:CYS79 3.5 14.2 1.0
CB C:CYS593 3.7 12.8 1.0
N C:CYS79 3.7 14.5 1.0
C2 C:FCO703 4.0 13.0 1.0
C1 C:FCO703 4.0 13.6 1.0
CA C:CYS79 4.2 13.0 1.0
NH1 C:ARG523 4.2 14.1 1.0
CB C:VAL78 4.4 14.9 1.0
CA C:CYS76 4.5 12.7 1.0
CZ C:ARG523 4.5 11.6 1.0
C3 C:FCO703 4.5 12.3 1.0
CA C:CYS590 4.5 13.5 1.0
N C:CYS593 4.6 11.7 1.0
CA C:CYS593 4.6 11.5 1.0
NE C:ARG523 4.6 11.8 1.0
CD C:ARG523 4.7 13.0 1.0
C C:VAL78 4.8 13.4 1.0
N2 C:FCO703 4.9 12.1 1.0
N C:VAL78 4.9 14.1 1.0
CA C:VAL78 4.9 14.1 1.0
N1 C:FCO703 5.0 13.5 1.0

Reference:

Y.Shomura, K.S.Yoon, H.Nishihara, Y.Higuchi. Structural Basis For A [4FE-3S] Cluster in the Oxygen-Tolerant Membrane-Bound [Nife]-Hydrogenase Nature V. 479 253 2011.
ISSN: ESSN 1476-4687
PubMed: 22002607
DOI: 10.1038/NATURE10504
Page generated: Thu Oct 10 08:16:29 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy